Laboratory Diagnostic Center, University Hospital RWTH Aachen, Aachen, Germany.
Institute of Molecular Pathobiochemistry, Experimental Gene Therapy and Clinical Chemistry, Aachen, Germany.
Clin Endocrinol (Oxf). 2019 Jun;90(6):827-833. doi: 10.1111/cen.13967. Epub 2019 Apr 16.
Anti-Mullerian hormone (AMH) together with luteinizing hormone (LH) and follicle-stimulating hormone (FSH) plays crucial roles in gonadal functions. However, the possible effects of GnRH on AMH via the hypothalamic-pituitary-gonadal (HPG) axis remain unexplored. We aimed to explore the changes in AMH levels after bolus GnRH stimulation and understand the relationship of AMH with FSH and LH in healthy subjects.
Thirty-one prepubertal children (15 males/16 females) and 78 adults (36 males/42 females) were included. We collected basal (0 minute) samples for determining levels of hormones. After GnRH treatment at a dose of 2.5 μg/kg body weight (maximum of 100 μg/kg body weight) intravenously, blood was collected at 30 minutes intervals for 120 minutes. Serum LH, FSH and AMH were measured by electrochemiluminometric assays.
After injection of GnRH, AMH levels were significantly decreased in 30 minutes (P < 0.001) in all groups with parallel increase of FSH and LH. In the second 30 minutes, all hormones levels reversed. There was also a moderate correlation between AMH and FSH (r = -0.430, P < 0.001).
GnRH lowers serum AMH levels, which have a negative correlation with the increase in gonadotrophins. These data pinpoint GnRH as an important factor of the AMH regulation, leading new opportunities for the understanding of AMH role in reproductive function and dysfunction.
抗缪勒管激素（AMH）与促黄体生成素（LH）和卵泡刺激素（FSH）一起在性腺功能中起关键作用。然而，促性腺激素释放激素（GnRH）通过下丘脑-垂体-性腺（HPG）轴对 AMH 的可能影响仍未被探索。我们旨在研究促性腺激素释放激素刺激后 AMH 水平的变化，并了解健康受试者中 AMH 与 FSH 和 LH 的关系。
纳入 31 名青春期前儿童（15 名男性/16 名女性）和 78 名成年人（36 名男性/42 名女性）。我们采集基础（0 分钟）样本以确定激素水平。在静脉内给予 2.5μg/kg 体重（最大 100μg/kg 体重）剂量的 GnRH 后，在 120 分钟的时间间隔内每隔 30 分钟采集血液。通过电化学发光免疫测定法测量血清 LH、FSH 和 AMH。
在 GnRH 注射后，所有组的 AMH 水平在 30 分钟时显著降低（P<0.001），同时 FSH 和 LH 水平升高。在接下来的 30 分钟内，所有激素水平都发生了逆转。AMH 与 FSH 之间也存在中度相关性（r=-0.430，P<0.001）。
GnRH 降低血清 AMH 水平，与促性腺激素的增加呈负相关。这些数据表明 GnRH 是 AMH 调节的一个重要因素，为理解 AMH 在生殖功能和功能障碍中的作用提供了新的机会。
