Chiesa Luca Maria, Nobile Maria, Panseri Sara, Arioli Francesco
a Department of Health , Animal Science and Food Safety, University of Milan , Milan , Italy.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2019 Apr;36(4):592-600. doi: 10.1080/19440049.2019.1583380. Epub 2019 Mar 14.
Glyphosate and glufosinate are broad spectrum herbicides, widely used in agriculture and in inhabited or industrialised areas, and aminomethylphosphonic acid is a degradation product of glyphosate. In 2015, the International Agency for Research on Cancer reported that glyphosate is a probable carcinogenic. In 2017, however, a scientific opinion of the European Chemicals Agency concluded that glyphosate is not proven to be carcinogenic, mutagenic or to have negative effects on reproduction. Nevertheless, aminomethylphosphonic acid was not considered. Due to their chemical-physical characteristics, these molecules present difficulties that have not yet allowed routine monitoring to be carried out. For these reasons, we developed and validated a simple and versatile liquid extraction, before IC-HRMS analysis, of three different complex matrices: honey, bass fish and bovine muscle. Among the satisfactory validation parameters, the LOQs in the range of 4.30-9.26 ng g demonstrated high method sensitivity, compared to the few works present in literature. Finally, the method was applied to real commercial samples, which showed no traces of the selected pesticides.
草甘膦和草铵膦是广谱除草剂，广泛应用于农业以及有人居住或工业化地区，氨甲基膦酸是草甘膦的降解产物。2015年，国际癌症研究机构报告称草甘膦可能致癌。然而，2017年欧洲化学品管理局的一项科学意见得出结论，草甘膦未被证明具有致癌性、致突变性或对生殖有负面影响。不过，氨甲基膦酸未被纳入考量。由于其物理化学特性，这些分子存在一些困难，尚未能进行常规监测。基于这些原因，我们开发并验证了一种简单且通用的液体萃取方法，用于在离子色谱-高分辨质谱分析之前，从蜂蜜、鲈鱼和牛肌肉这三种不同的复杂基质中提取目标物。在令人满意的验证参数中，4.30 - 9.26 ng/g的定量限表明该方法具有较高的灵敏度，相比文献中为数不多的相关研究而言。最后，该方法应用于实际商业样品，结果显示未检测到所选农药的痕迹。
