Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Northwest University, Xi'an 710069, China.
Shaanxi Pharmaceutical Holding Group Co., Ltd., Xi'an 710069, China.
Int J Mol Sci. 2019 Mar 13;20(6):1267. doi: 10.3390/ijms20061267.
miRNAs play vital regulatory roles in different plant developmental stages and in plant response to biotic and abiotic stresses. However, information is limited on the miRNA regulatory mechanism to methyl jasmonate (MeJA). In this study, we used the microRNAome profile to illustrate the relevant regulatory mechanisms of in response to methyl jasmonate (MeJA) through Illumina RNA-Seq. As a result, we identified 875 miRNAs corresponding to 11,277 target mRNAs, among them, 168 known miRNA families representing 6019 target mRNAs sequences were obtained. 452 miRNA-mRNA pairs presented an anti-correlationship (Cor < -0.50 and -value of correlation ≤ 0.05). The miRNA with a fold change ≥ 2 and a (-Value) < 0.05 in pairwise comparison were identified as significant differentially expressed miRNAs (DEMs). The DEMs in MeJA treatment of 0, 24, 36 and 48 h were compared by using Short Time Expression Miner (STEM) cluster and 4 significant gene profiles (-value ≤ 0.02) were identified. Through the kyoto encyclopedia of genes and genomes (KEGG) pathway and gene ontology (GO) enrichment analysis on all miRNA targets, we identified 33 mRNAs in terpenoid biosynthesis, which were regulated by miRNAs under MeJA treatment, so the miRNA maybe involved in the response of plant to exogenous MeJA and the results would provide very useful information on illustrating the regulatory mechanism of and also provide an overall view of the miRNAs response to MeJA stress of a non-model plant.
miRNAs 在植物不同发育阶段和植物对生物和非生物胁迫的反应中发挥着重要的调节作用。然而，关于 miRNA 对甲基茉莉酸（MeJA）的调节机制的信息有限。在这项研究中，我们使用 microRNAome 图谱通过 Illumina RNA-Seq 来阐明 对甲基茉莉酸（MeJA）的反应的相关调节机制。结果，我们鉴定了 875 个对应于 11277 个靶 mRNAs 的 miRNAs，其中获得了 168 个已知的 miRNA 家族，代表 6019 个靶 mRNAs 序列。452 个 miRNA-mRNA 对呈现反相关（Cor < -0.50 和 -值的相关性 ≤ 0.05）。miRNA 的倍数变化≥2 和 (-值) < 0.05 在成对比较中被鉴定为显著差异表达的 miRNAs (DEMs)。通过短时间表达 Miner (STEM) 聚类比较 MeJA 处理 0、24、36 和 48 h 的 DEMs，鉴定了 4 个显著的基因谱（-值≤0.02）。通过对所有 miRNA 靶标进行京都基因与基因组百科全书 (KEGG) 通路和基因本体论 (GO) 富集分析，我们鉴定了萜类生物合成中的 33 个 mRNAs，它们受 MeJA 处理下的 miRNAs 调节，因此 miRNA 可能参与 植物对外源 MeJA 的反应，研究结果将为阐明 调节机制提供非常有用的信息，并为非模式植物对 MeJA 胁迫的 miRNA 反应提供全面的了解。
