College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, China, 350002; School of Food Science and Engineering, South China University of Technology, Guangzhou, China, 510641.
College of Food Science, Fujian Agriculture and Forestry University, Fuzhou, China, 350002.
Int J Biol Macromol. 2019 Jun 15;131:209-217. doi: 10.1016/j.ijbiomac.2019.03.061. Epub 2019 Mar 11.
Microspheres play an increasingly important role in the food and medicine industries. In this study, konjac glucomannan (KGM)/sodium alginate (SA)/graphene oxide (GO) solution was injected into CaCl solution under high-voltage static electricity assistance to fabricate microspheres. Then, chitosan (CS) was coated on the surface of the microspheres to enhance their stability. SEM images confirmed that increasing voltage decreased the particle size of microspheres obviously. Furthermore, GO was beneficial in maintaining the full structure of freeze-dried microspheres, and the CS membrane improved the surface of the microspheres with no relatively obvious gully. Results indicated that KGM interacted with SA by hydrogen bond, and GO improved this interaction in microspheres. Furthermore, swelling tests showed that the microspheres exhibited different swelling properties in different media, and the CS membrane could improve the stability of microspheres in simulated intestinal fluid and simulated colon fluids. Moreover, GO could greatly improve the ciprofloxacin (CPFX) loading efficiency of microspheres, and achieving a sustained release effect of CPFX. Thus, CS-coated KGM/SA/GO microspheres showed great potential application in drug and/or nutrition factor colon-targeted delivery.
微球在食品和医药行业中发挥着越来越重要的作用。在这项研究中，通过高压静电辅助将魔芋葡甘聚糖（KGM）/海藻酸钠（SA）/氧化石墨烯（GO）溶液注入到 CaCl2 溶液中，制备了微球。然后，在微球表面包覆壳聚糖（CS）以提高其稳定性。SEM 图像证实，增加电压明显降低了微球的粒径。此外，GO 有利于保持冻干微球的完整结构，CS 膜改善了微球表面，没有明显的沟壑。结果表明，KGM 通过氢键与 SA 相互作用，GO 改善了微球中的这种相互作用。此外，溶胀试验表明，微球在不同介质中表现出不同的溶胀性能，CS 膜可以提高微球在模拟肠液和模拟结肠液中的稳定性。此外，GO 可以大大提高微球对环丙沙星（CPFX）的载药效率，并实现 CPFX 的持续释放效果。因此，CS 包覆的 KGM/SA/GO 微球在药物和/或营养因子结肠靶向递药方面具有很大的应用潜力。
