使用叶提取物合成的银纳米颗粒对……和……的抗菌作用
antibacterial effects of silver nanoparticles synthesized using leaf extract on and .
作者信息
Sanchooli Narjes, Saeidi Saeide, Barani Hashem Khandan, Sanchooli Esmael
机构信息
Department of Fisheries of Hamoun International Wetland Research Institute, University of Zabol, Zabol, Iran.
Zabol Medicinal Plant Research Center, Zabol University of Medical Sciences, Zabol, Iran.
出版信息
Iran J Microbiol. 2018 Dec;10(6):400-408.
BACKGROUND AND OBJECTIVES
The use of plants for the synthesis of nanoparticles has received attention. The present study aimed to evaluate the antibacterial effects of silver nanoparticles synthesized by leaf extract against and .
MATERIALS AND METHODS
Silver nanoparticles were obtained by reacting silver nitrate solution 2 mM and leaf extract. The AgNPs were characterized by UV-visible spectrophotometer, scanning electron microscopy (SEM), and Fourier transform infrared spectrometer (FTIR). To determine minimum inhibitory concentration and test antibiogram of nanoparticle synthesized, broth micro dilution and agar well diffusion methods were used, respectively.
RESULTS
The zones of bacterial inhibition were 16 ± 0.5 and 9.16 ± 0.28 mm against and using 10 and 0.62 mg/mL AgNPs, respectively. Among the studied bacterial species, silver nanoparticles were more effective on and and less effective on . The highest MIC and MBC of AgNPs (2.5 and 5 mg/mL) were observed for . The lowest MIC and MBC of AgNPs (0.32 and 0.62 mg/mL) were observed for , respectively. The MIC and MBC of AgNPs were found to be 1.25 and 2.5 mg/mL for .
CONCLUSION
The results clearly indicated that AgNPs have potential antimicrobial activity against Gram-positive and Gram-negative bacteria.
背景与目的
利用植物合成纳米颗粒已受到关注。本研究旨在评估由叶提取物合成的银纳米颗粒对[具体细菌1]和[具体细菌2]的抗菌效果。
材料与方法
通过将2 mM硝酸银溶液与叶提取物反应获得银纳米颗粒。利用紫外可见分光光度计、扫描电子显微镜（SEM）和傅里叶变换红外光谱仪（FTIR）对银纳米颗粒进行表征。分别采用肉汤微量稀释法和琼脂孔扩散法测定合成纳米颗粒的最低抑菌浓度并测试其抗菌谱。
结果
使用10 mg/mL和0.62 mg/mL的银纳米颗粒时，对[具体细菌1]和[具体细菌2]的抑菌圈分别为16±0.5和9.16±0.28 mm。在所研究的细菌种类中，银纳米颗粒对[具体细菌3]和[具体细菌4]更有效，对[具体细菌5]效果较差。银纳米颗粒对[具体细菌6]的最高最低抑菌浓度（MIC）和最低杀菌浓度（MBC）为2.5和5 mg/mL。银纳米颗粒对[具体细菌7]的最低MIC和MBC分别为0.32和0.62 mg/mL。银纳米颗粒对[具体细菌8]的MIC和MBC分别为1.25和2.5 mg/mL。
结论
结果清楚地表明，[植物名称]银纳米颗粒对革兰氏阳性菌和革兰氏阴性菌具有潜在的抗菌活性。
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