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使用新型均匀磁场提高磁性聚乙烯亚胺纳米颗粒对MG-63成骨细胞的磁转染效率

Improving Magnetofection of Magnetic Polyethylenimine Nanoparticles into MG-63 Osteoblasts Using a Novel Uniform Magnetic Field.

作者信息

Cen Chaode, Wu Jun, Zhang Yong, Luo Cong, Xie Lina, Zhang Xin, Yang Xiaolan, Li Ming, Bi Yang, Li Tingyu, He Tongchuan

机构信息

Department of Orthopedics, Guizhou Provincial Orthopedics Hospital, Guiyang, 550000, People's Republic of China.

Department of Orthopedics, Laboratory of Orthopedic Biomaterials, Children's Hospital of Chongqing Medical University, Chongqing, 400014, People's Republic of China.

出版信息

Nanoscale Res Lett. 2019 Mar 12;14(1):90. doi: 10.1186/s11671-019-2882-5.

DOI:10.1186/s11671-019-2882-5
PMID:30874913
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6419855/
Abstract

This study aimed to improve the magnetofection of MG-63 osteoblasts by integrating the use of a novel uniform magnetic field with low molecular weight polyethylenimine modified superparamagnetic iron oxide nanoparticles (PEI-SPIO-NPs). The excellent characteristics of PEI-SPIO-NPs such as size, zeta potential, the pDNA binding and protective ability were determined to be suitable for gene delivery. The novel uniform magnetic field enabled polyethylenimine-modified superparamagnetic iron oxide nanoparticles/pDNA complexes (PEI-SPIO-NPs/pDNA complexes) to rapidly and uniformly distribute on the surface of MG-63 cells, averting local transfection and decreasing disruption of the membrane caused by the centralization of positively charged PEI-SPIO-NPs, thereby increasing the effective coverage of magnetic gene carriers during transfection, and improving magnetofection efficiency. This innovative uniform magnetic field can be used to determine the optimal amount between PEI-SPIO-NPs and pDNA, as well as screen for the optimal formulation design of magnetic gene carrier under the homogenous conditions. Most importantly, the novel uniform magnetic field facilitates the transfection of PEI-SPIO-NPs/pDNA into osteoblasts, thereby providing a novel approach for the targeted delivery of therapeutic genes to osteosarcoma tissues as well as a reference for the treatment of other tumors.

摘要

本研究旨在通过将新型均匀磁场与低分子量聚乙烯亚胺修饰的超顺磁性氧化铁纳米颗粒（PEI-SPIO-NPs）结合使用，提高MG-63成骨细胞的磁转染效率。已确定PEI-SPIO-NPs的优异特性，如尺寸、zeta电位、与pDNA的结合能力和保护能力，适合用于基因递送。新型均匀磁场使聚乙烯亚胺修饰的超顺磁性氧化铁纳米颗粒/pDNA复合物（PEI-SPIO-NPs/pDNA复合物）能够快速且均匀地分布在MG-63细胞表面，避免局部转染，并减少带正电的PEI-SPIO-NPs集中导致的细胞膜破坏，从而在转染过程中增加磁性基因载体的有效覆盖范围，提高磁转染效率。这种创新的均匀磁场可用于确定PEI-SPIO-NPs与pDNA之间的最佳用量，以及在均匀条件下筛选磁性基因载体的最佳配方设计。最重要的是，新型均匀磁场有助于将PEI-SPIO-NPs/pDNA转染到成骨细胞中，从而为将治疗性基因靶向递送至骨肉瘤组织提供了一种新方法，并为其他肿瘤的治疗提供参考。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c364/6419855/74d32112aef3/11671_2019_2882_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c364/6419855/92b45b2fef62/11671_2019_2882_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c364/6419855/c381c9d11a8d/11671_2019_2882_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c364/6419855/0b49ef095118/11671_2019_2882_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c364/6419855/5f9254b203b6/11671_2019_2882_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c364/6419855/8ec2c7b49551/11671_2019_2882_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c364/6419855/a19eec8745f8/11671_2019_2882_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c364/6419855/74d32112aef3/11671_2019_2882_Fig7_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c364/6419855/92b45b2fef62/11671_2019_2882_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c364/6419855/c381c9d11a8d/11671_2019_2882_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c364/6419855/0b49ef095118/11671_2019_2882_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c364/6419855/5f9254b203b6/11671_2019_2882_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c364/6419855/8ec2c7b49551/11671_2019_2882_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c364/6419855/a19eec8745f8/11671_2019_2882_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c364/6419855/74d32112aef3/11671_2019_2882_Fig7_HTML.jpg

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