Brake Marisa A, Ivanciu Lacramioara, Maroney Susan A, Martinez Nicolas D, Mast Alan E, Westrick Randal J
Department of Biological Sciences, Oakland University, Rochester, Michigan.
Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania.
Curr Protoc Mouse Biol. 2019 Jun;9(2):e61. doi: 10.1002/cpmo.61. Epub 2019 Mar 15.
The mammalian blood coagulation system was designed to restrict blood loss due to injury as well as keep the blood fluid within the blood vessels of the organism. Blood coagulation activity in inbred mouse strains varies widely among strains, suggesting that many genomic variants affect hemostasis. Some of these molecules have been discovered and characterized; however, many are still unknown. Genetically modified mouse technologies are providing a plethora of new mouse models for investigating the regulation of blood coagulation. Here we provide a protocol for the tail bleeding time as a primary assessment of in vivo blood coagulation, as well as in vitro methods such as the prothrombin time, activated partial thromboplastin time, and thrombin generation assay. We also provide protocols for the assessment of the activities of specific known factors involved in blood coagulation. © 2019 by John Wiley & Sons, Inc.
哺乳动物的血液凝固系统旨在限制因受伤导致的失血，并使血液在生物体的血管内保持流动状态。近交系小鼠品系中的血液凝固活性在不同品系间差异很大，这表明许多基因组变异会影响止血功能。其中一些分子已被发现并进行了特征描述；然而，仍有许多未知。基因编辑小鼠技术为研究血液凝固的调控提供了大量新的小鼠模型。在此，我们提供了一项用于评估体内血液凝固的主要指标——尾部出血时间的实验方案，以及一些体外实验方法，如凝血酶原时间、活化部分凝血活酶时间和凝血酶生成测定。我们还提供了评估参与血液凝固的特定已知因子活性的实验方案。© 2019约翰威立国际出版公司
