Department of Chemical & Biomolecular Engineering, Clemson University, Clemson, SC 29634, United States.
Department of Chemical & Biomolecular Engineering, Clemson University, Clemson, SC 29634, United States.
Curr Opin Biotechnol. 2019 Jun;57:73-81. doi: 10.1016/j.copbio.2019.02.011. Epub 2019 Mar 12.
Current transportation fuels derived from petroleum can also be made from microbial systems. In particular, oleaginous yeast have naturally evolved high flux pathways for fatty acids in the form of neutral lipids, which can be converted into a variety of drop-in fuels. Here, we describe the recent advances in the use of the four most popular oleaginous yeasts for making lipids and other potential fuels - Yarrowia lipolytica, Lipomyces starkeyi, Rhodosporidium toruloides, and Cutaneotrichosporon oleaginosus. The paper is divided into three major sections focusing on (1) the important natural complex phenotypes of each yeast; (2) the development of metabolic engineering tools for each yeast; and (3) demonstrations of metabolic engineering in each yeast. At the end of each section, we provide our assessment, of which yeast is most promising in the near and long term for bioenergy production.
当前源自石油的运输燃料也可以通过微生物系统来生产。具体来说，产油酵母自然进化出了高流量的脂肪酸途径，以中性脂质的形式存在，这些脂质可以转化为各种可替代的燃料。在这里，我们描述了最近在利用四种最受欢迎的产油酵母生产油脂和其他潜在燃料方面的进展——解脂耶氏酵母、斯达氏油脂酵母、粘红酵母和脆壁克鲁维酵母。本文分为三个主要部分，重点介绍：（1）每种酵母的重要天然复杂表型；（2）每种酵母代谢工程工具的开发；（3）每种酵母的代谢工程演示。在每个部分的结尾，我们对哪种酵母在近期和长期内最有希望用于生物能源生产进行了评估。
