Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.
Department of Biotechnology, Indian Institute of Technology Kharagpur, Kharagpur 721302, India.
Biotechnol Adv. 2021 Dec;53:107722. doi: 10.1016/j.biotechadv.2021.107722. Epub 2021 Feb 23.
With the increasing demand to develop a renewable and sustainable biolipid feedstock, several species of non-conventional oleaginous yeasts are being explored. Apart from the platform oleaginous yeast Yarrowia lipolytica, the understanding of metabolic pathway and, therefore, exploiting the engineering prospects of most of the oleaginous species are still in infancy. However, in the past few years, enormous efforts have been invested in Rhodotorula, Rhodosporidium, Lipomyces, Trichosporon, and Candida genera of yeasts among others, with the rapid advancement of engineering strategies, significant improvement in genetic tools and techniques, generation of extensive bioinformatics and omics data. In this review, we have collated these recent progresses to make a detailed and insightful summary of the major developments in metabolic engineering of the prominent oleaginous yeast species. Such a comprehensive overview would be a useful resource for future strain improvement and metabolic engineering studies for enhanced production of lipid and lipid-derived chemicals in oleaginous yeasts.
随着对开发可再生和可持续生物脂质原料的需求不断增加，几种非常规产油酵母正在被探索。除了平台产油酵母解脂耶氏酵母（Yarrowia lipolytica）之外，大多数产油酵母的代谢途径的理解，因此，其工程前景的开发仍处于起步阶段。然而，在过去的几年中，人们投入了巨大的努力来研究酵母属中的红酵母（Rhodotorula）、红冬孢酵母（Rhodosporidium）、类丝孢酵母（Lipomyces）、毕赤酵母（Trichosporon）和假丝酵母（Candida）等，随着工程策略的快速发展，遗传工具和技术的显著改进，以及广泛的生物信息学和组学数据的产生。在这篇综述中，我们收集了这些最新进展，对主要产油酵母的代谢工程的重大发展进行了详细而有见地的总结。这样一个全面的综述将是未来菌株改良和代谢工程研究的有用资源，以提高产油酵母中脂质和脂质衍生化学品的产量。
