唾液 microRNAs 检测反映慢性牙周炎：一项初步研究。
Detection of Salivary miRNAs Reflecting Chronic Periodontitis: A Pilot Study.
机构信息
Department of Preventive Dentistry, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Kita-ku, Okayama 700-8558, Japan.
Department of Community Oral Health, Asahi University School of Dentistry, 1851-1 Hozumi, Mizuho, Gifu 501-0296, Japan.
出版信息
Molecules. 2019 Mar 15;24(6):1034. doi: 10.3390/molecules24061034.
The purpose of this cross-sectional pilot study was to find salivary microRNAs (miRNAs) reflecting periodontal condition in chronic periodontitis. One hundred and twenty chronic periodontitis patients (mean age, 68.4 years) participated in the study, from whom unstimulated whole saliva was collected. A multiphase study was conducted to explore salivary miRNAs as biomarkers of periodontitis. At first, a polymerase chain reaction (PCR) array was performed to compare salivary miRNAs profiles in no and mild (no/mild) and severe periodontitis patients. Next, the relative expression of salivary miRNAs on individual samples was assessed by real-time reverse transcription-PCR. The numbers (%) of patients were 26 (21.6%, no/mild), 58 (48.3%, moderate) and 36 (30.0%, severe), respectively. Among 84 miRNAs, only the relative expression of hsa-miR-381-3p in the severe periodontitis group was significantly higher than that of the no/mild periodontitis group ( < 0.05). Among the 120 patients, there was also a significant correlation between the relative expression of hsa-miR-381-3p and the mean probing pocket depth (PPD) (r = 0.181, < 0.05). Salivary hsa-miR-381-3p was correlated with periodontitis condition in chronic periodontitis patients.
本横断面初步研究的目的是寻找反映慢性牙周炎牙周状况的唾液 microRNAs（miRNAs）。120 例慢性牙周炎患者（平均年龄 68.4 岁）参与了这项研究，从中采集了未刺激的全唾液。进行了多阶段研究以探索唾液 miRNAs 作为牙周炎生物标志物。首先，通过聚合酶链反应（PCR）阵列比较了无和轻度（无/轻度）和重度牙周炎患者的唾液 miRNAs 谱。接下来，通过实时逆转录-PCR 评估了个体样本中唾液 miRNAs 的相对表达。患者的数量（%）分别为 26（21.6%，无/轻度），58（48.3%，中度）和 36（30.0%，重度）。在 84 种 miRNA 中，只有重度牙周炎组 hsa-miR-381-3p 的相对表达明显高于无/轻度牙周炎组（<0.05）。在 120 例患者中，hsa-miR-381-3p 的相对表达与平均探诊袋深度（PPD）之间也存在显著相关性（r=0.181，<0.05）。唾液 hsa-miR-381-3p 与慢性牙周炎患者的牙周炎状况相关。
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