Southeast Asia Disaster Prevention Research Initiative (SEADPRI-UKM), Institute for Environment and Development (LESTARI), Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia.
School of Chemical Sciences and Food Technology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM Bangi, Selangor Darul Ehsan, Malaysia.
Talanta. 2019 Jun 1;198:358-370. doi: 10.1016/j.talanta.2019.02.036. Epub 2019 Feb 6.
An optical genosensor based on Schiff base complex (Zn salphen) DNA label and acrylic microspheres (AMs) as polymer support of the capturing DNA probe (cpDNA) was developed for dengue virus serotype 2 (DEN-2) detection via reflectance spectrophotometric method. The solid-state optical DNA biosensor showed high selectivity and specificity up to one-base mismatch in the target DNA sequence owing to the salphen chemical structure that is rich in localized electrons, and allowed π-π stacking interaction between stacked base pairs of double-stranded DNA (dsDNA). The reflectometric DNA microsensor demonstrated a broad linear detection range towards DEN-2 DNA from 1 × 10 M to 1 × 10 M with a low limit of detection (LOD) obtained at 1.21 × 10 M. The DNA biosensor gave reproducible optical response with a satisfactory relative standard deviation (RSD) at 3.1%, (n = 3), and the reflectance response was stable even after four regeneration cycles of the DNA biosensor. The optical genosensor was proven comparable with standard reverse transcription polymerase chain reaction (RT-PCR) in detecting DEN-2 genome acquired from clinical samples of serum, urine and saliva of dengue virus infected patients under informed consent. The developed reflectometric DNA biosensor is advantageous in offering an early DEN-2 diagnosis, when fever symptom started to manifest in patient.
基于席夫碱配合物（Zn salphen）DNA 标记和丙烯微球（AMs）作为捕获 DNA 探针（cpDNA）的聚合物支持物的光学基因传感器已被开发用于通过反射分光光度法检测登革热病毒血清型 2（DEN-2）。由于席夫碱化学结构富含局部电子，允许双链 DNA（dsDNA）堆叠碱基对之间发生 π-π 堆积相互作用，因此固态光学 DNA 生物传感器对靶 DNA 序列中的单个碱基错配具有高选择性和特异性。反射式 DNA 微传感器对 DEN-2 DNA 表现出宽线性检测范围，从 1×10 M 到 1×10 M，检测下限（LOD）低至 1.21×10 M。该 DNA 生物传感器具有可重复的光学响应，相对标准偏差（RSD）为 3.1%（n=3），并且即使在 DNA 生物传感器经过四次再生循环后，反射率响应仍然稳定。光学基因传感器与标准逆转录聚合酶链反应（RT-PCR）在检测知情同意下从登革热病毒感染患者的血清、尿液和唾液中获得的 DEN-2 基因组方面具有可比性。所开发的反射式 DNA 生物传感器在患者出现发热症状时具有早期 DEN-2 诊断的优势。
