假定非致病性单核细胞增生李斯特菌菌株的溶血活性重新评估
Hemolytic activity reevaluation of putative nonpathogenic Listeria monocytogenes strains.
作者信息
Lachica R V
机构信息
U.S. Army Solider Systems Command, Natick Research, Development, and Engineering Center, Massachusetts 01760, USA.
出版信息
Appl Environ Microbiol. 1996 Nov;62(11):4293-5. doi: 10.1128/aem.62.11.4293-4295.1996.
Identification of 12 strains originally characterized as nonpathogenic Listeria monocytogenes was reassured following the evaluation of their hemolytic capability with a newly developed horse blood agar plate. Seven of the strains were observed consistently to be hemolytic and confirmed as L. monocytogenes with the use of two commercial systems: the Gene-Trak L. monocytogenes-specific colorimetric DNA hybridization assay and the API Listeria system. Except for one strain that formed typical smooth colonies, these hemolytic strains formed rough colonies on a selective medium, lithium chloride-ceftazidime agar. The rest of the strains were nonhemolytic and did not hybridize with the DNA probe; they were identified as Listeria innocua on the basis of their API Listeria system biochemical profile. All but one of these nonhemolytic strains formed smooth colonies on lithium chloride-ceftazidime agar.
在使用新开发的马血琼脂平板评估了12株最初被鉴定为非致病性单核细胞增生李斯特菌菌株的溶血能力后，重新确定了它们的身份。观察到其中7株菌株始终具有溶血能力，并通过两种商业系统确认为单核细胞增生李斯特菌：Gene-Trak单核细胞增生李斯特菌特异性比色DNA杂交检测法和API李斯特菌系统。除了一株形成典型光滑菌落的菌株外，这些溶血菌株在选择性培养基氯化锂-头孢他啶琼脂上形成粗糙菌落。其余菌株无溶血能力，且不与DNA探针杂交；根据其API李斯特菌系统生化图谱，它们被鉴定为无害李斯特菌。除了其中一株非溶血菌株外，所有这些非溶血菌株在氯化锂-头孢他啶琼脂上均形成光滑菌落。
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