Boyle W J, Lipsick J S, Baluda M A
Proc Natl Acad Sci U S A. 1986 Jul;83(13):4685-9. doi: 10.1073/pnas.83.13.4685.
Antibodies directed against a bacterial fusion protein that contains the domain encoded by the highly evolutionarily conserved 5' one-third of the v-myb oncogene of avian myeloblastosis virus (AMV) detect the protein products of various members of the myb gene family. Immunoprecipitation or immunoblot analyses with these antibodies yielded the following information. First, the products of the v-myb oncogenes of AMV (p48v-myb) and of E26 virus (p135gag-myb-ets) contain this highly conserved amino acid sequence, as previously hypothesized. Second, p75c-myb, the product of the chicken c-myb protooncogene, also contains this protein domain. Third, these antibodies have identified the products of the human, murine, and Drosophila c-myb genes, which were all found to be nuclear proteins of Mr 75,000-80,000. The human c-myb protein product is present in immature cells of the erythroid, myeloid, and lymphoid lineages.
针对一种细菌融合蛋白的抗体可检测到myb基因家族各成员的蛋白质产物，该细菌融合蛋白含有由禽成髓细胞瘤病毒（AMV）的v-myb癌基因高度进化保守的5'三分之一编码的结构域。用这些抗体进行免疫沉淀或免疫印迹分析得到了以下信息。首先，如先前假设的那样，AMV的v-myb癌基因（p48v-myb）和E26病毒的v-myb癌基因（p135gag-myb-ets）的产物含有这种高度保守的氨基酸序列。其次，鸡c-myb原癌基因的产物p75c-myb也含有这种蛋白质结构域。第三，这些抗体鉴定出了人类、小鼠和果蝇c-myb基因的产物，它们均为分子量75,000 - 80,000的核蛋白。人类c-myb蛋白产物存在于红细胞系、髓细胞系和淋巴细胞系的未成熟细胞中。
