Urbánek P, Dvorák M, Bartunĕk P, Pecenka V, Paces V, Trávnícek M
Institute of Molecular Genetics, Czechoslovak Academy of Sciences, Prague.
Nucleic Acids Res. 1988 Dec 23;16(24):11521-30. doi: 10.1093/nar/16.24.11521.
The nucleotide sequence of the chicken myb proto-oncogene putative promoter region was determined and compared with the corresponding sequence of the mouse c-myb gene (1). 118 bp upstream from the initiation codon suggested by Gerondakis and Bishop (2) for the chicken c-myb protein, a 124-bp-long conserved element was found (92% identity in chicken and mouse sequences). Sequences homologous to this element were detected on Southern blots of restricted genomic DNAs from mouse, man, lizard, frog, and carp. No hybridization was observed with Drosophila, yeast, or Escherichia coli DNA. In human DNA, sequences homologous to this element were located at the 5' end of the c-myb gene, i.e. in the same position as in the chicken and mouse genes. Several lines of evidence suggest that the element is not a coding exon of a gene overlapping the c-myb gene. It may be of importance that one of the DNase I-sensitive sites and several c-myb mRNA cap sites localized recently in the mouse c-myb gene (3,4) lie within this region. It is suggested that this evolutionarily conserved element is involved in the regulation of myb proto-oncogene expression in vertebrates.
测定了鸡myb原癌基因假定启动子区域的核苷酸序列，并与小鼠c-myb基因的相应序列进行了比较(1)。在Gerondakis和Bishop(2)所建议的鸡c-myb蛋白起始密码子上游118 bp处，发现了一个124 bp长的保守元件(鸡和小鼠序列的同源性为92%)。在来自小鼠、人、蜥蜴、青蛙和鲤鱼的限制性基因组DNA的Southern印迹上检测到与该元件同源的序列。未观察到与果蝇、酵母或大肠杆菌DNA的杂交。在人类DNA中，与该元件同源的序列位于c-myb基因的5'端，即在与鸡和小鼠基因相同的位置。几条证据表明该元件不是与c-myb基因重叠的基因的编码外显子。最近在小鼠c-myb基因(3,4)中定位的一个DNase I敏感位点和几个c-myb mRNA帽位点位于该区域内，这可能很重要。有人提出，这个进化上保守的元件参与了脊椎动物中myb原癌基因表达的调控。
