Liou Jong-Shian, Huang Chien-Hsun, Wang Chun-Lin, Lee Ai-Yun, Mori Koji, Tamura Tomohiko, Watanabe Mizuki, Blom Jochen, Huang Lina, Watanabe Koichi
1Bioresource Collection and Research Center, Food Industry Research and Development Institute, 331 Shih-Pin Rd, Hsinchu 30062, Taiwan, ROC.
2Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE), 2-5-8, Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
Int J Syst Evol Microbiol. 2019 May;69(5):1484-1489. doi: 10.1099/ijsem.0.003346. Epub 2019 Mar 19.
A Gram-stain-positive, rod-shaped, non-motile, catalase-negative and facultative anaerobic strain, L88, was isolated from suan-tsai, a traditional Taiwanese fermented mustard green. Comparative analyses of 16S rRNA, pheS and rpoA gene sequences demonstrated that strain L88was a member of the genus Lactobacillus. On the basis of 16S rRNA gene sequence similarity, the type strains of Lactobacillus acidifarinae (98.2 % similarity), Lactobacillus namurensis (98.1 %), Lactobacillus zymae (98.1 %) and Lactobacillus spicheri (96.8 %) were the closest neighbours to this novel strain. The average nucleotide identity, digital DNA‒DNA hybridization and average amino acid identity values between L88 and its closest relatives were lower than 80, 30 and 90 %, respectively. Phenotypic and genotypic test results demonstrated that strain L88 represents a novel species of the genus Lactobacillus, for which the name Lactobacillus suantsaii sp. nov., is proposed. The type strain is L88 (=BCRC 12945=NBRC 113535).
从传统台湾发酵芥菜“酸菜”中分离出一株革兰氏阳性、杆状、无运动性、过氧化氢酶阴性兼性厌氧菌株L88。对16S rRNA、pheS和rpoA基因序列的比较分析表明，菌株L88是乳杆菌属的成员。基于16S rRNA基因序列相似性，嗜酸乳杆菌（相似性98.2%）、那慕尔乳杆菌（98.1%）、酶解乳杆菌（98.1%）和斯氏乳杆菌（96.8%）的模式菌株是该新菌株的最接近亲缘种。L88与其最亲近亲缘种之间的平均核苷酸同一性、数字DNA-DNA杂交和平均氨基酸同一性值分别低于80%、30%和9和90%。表型和基因型测试结果表明，菌株L88代表乳杆菌属的一个新物种，为此提出名称为嗜酸乳杆菌新种（Lactobacillus suantsaii sp. nov.）。模式菌株为L88（=BCRC 12945=NBRC 113535）。
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