Guu Jia-Rong, Wang Li-Ting, Hamada Moriyuki, Wang Chihuei, Lin Ru-Wei, Huang Lina, Watanabe Koichi
1Bioresource Collection and Research Center (BCRC), Food Industry Research and Development Institute, 331 Shih-Pin Rd., Hsinchu 30062, Taiwan, ROC.
2Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
Int J Syst Evol Microbiol. 2018 Aug;68(8):2424-2430. doi: 10.1099/ijsem.0.002837. Epub 2018 Jun 19.
A Gram-stain-positive strain, BS-W1, was isolated from a traditional fermented ma bamboo shoots (Dendrocalamus latiflorus Munro) product of Taiwan. It was rod-shaped, non-motile, non-haemolytic, asporogenous, facultatively anaerobic, heterofermentative and did not exhibit catalase or oxidase activities. Comparative analysis of 16S rRNA, pheS, rpoA and gyrB gene sequences demonstrated that the novel strain BS-W1 was a member of the genus Lactobacillus. On the basis of 16S RNA gene sequence similarity, the type strains of Lactobacillus oryzae (94.4 % similarity), Lactobacillus acidifarinae (93.8 %), Lactobacillus namurensis (93.7 %) and Lactobacillus zymae (93.7 %) were the closest neighbours to strain BS-W1. The pheS, rpoA and gyrB gene sequence similarities of strain BS-W1 to closely related these species were less than 80.2 %. DNA-DNA reassociation values with these type strains were 21.0-33.8 %. The DNA G+C content was 46.6 mol%. The average nucleotide identity values between BS-W1 and the closest relatives were lower than 70 %. Phenotypic and genotypic features demonstrated that the strain represents a novel species of the genus Lactobacillus, for which the name Lactobacillus bambusae sp. nov. is proposed. The type strain is BS-W1 (=BCRC 80970=NBRC 112377).
从台湾传统发酵麻竹笋（麻竹）产品中分离出一株革兰氏阳性菌株BS-W1。它呈杆状，无运动性，不溶血，不产芽孢，兼性厌氧，异型发酵，且不表现出过氧化氢酶或氧化酶活性。对16S rRNA、pheS、rpoA和gyrB基因序列的比较分析表明，新菌株BS-W1是乳杆菌属的成员。基于16S RNA基因序列相似性，米乳杆菌（相似性为94.4%）、嗜酸乳杆菌（93.8%）、那慕尔乳杆菌（93.7%）和酶解乳杆菌（93.7%）的模式菌株是与菌株BS-W1亲缘关系最近的邻居。菌株BS-W1与这些密切相关物种的pheS、rpoA和gyrB基因序列相似性均低于80.2%。与这些模式菌株的DNA-DNA重结合值为21.0-33.8%。DNA G+C含量为46.6 mol%。BS-W1与其最亲近亲缘种之间的平均核苷酸同一性值低于70%。表型和基因型特征表明，该菌株代表乳杆菌属的一个新物种，为此提议将其命名为竹乳杆菌（Lactobacillus bambusae sp. nov.）。模式菌株为BS-W1（=BCRC 80970=NBRC 112377）。
