sp. nov., sp. nov., sp. nov., sp. nov., sp. nov., sp. nov., sp. nov., sp. nov. 和 sp. nov.，均从中国传统泡菜和酸奶中分离得到。
sp. nov., sp. nov., sp. nov., sp. nov., sp. nov., sp. nov., sp. nov., sp. nov. and sp. nov., isolated from Chinese traditional pickle and yogurt.
机构信息
College of Life Sciences, Northeast Agricultural University, Harbin 150030, PR China.
出版信息
Int J Syst Evol Microbiol. 2019 Oct;69(10):3178-3190. doi: 10.1099/ijsem.0.003609.
Fourteen Gram-stain-positive bacterial strains were isolated from Chinese traditional pickle and yogurt. The strains were characterised using a polyphasic taxonomic approach, including 16S rRNA gene sequence analysis, gene sequence analysis, gene sequence analysis, determination of DNA G+C content, determination of average nucleotide identity, fatty acid methyl ester analysis and an analysis of phenotypic features. The data demonstrated that the fourteen strains represented nine novel species belonging to the genus , strains 54-2, 54-5, 33-7, 116-2, 184-8, 204-8, 8-1(1), 256-3 and M1575 were designated as the type strains. Strain 54-2 was phylogenetically related to the type strains of and , having 96.5 and 91.6 % 16S rRNA gene sequence similarities, less than 74.6 % gene sequence similarities, less than 81.6 % gene sequence similarities and less than 72.5 % ANI values. Strain 54-5 was phylogenetically related to the type strains of and , exhibiting 99.1 and 97.3 % 16S rRNA gene sequence similarities, less than 83.1 % gene sequence similarities, less than 93.1 % gene sequence similarities and less than 79.9 % ANI values. Strains 33-7, 116-2, 184-8, 204-8, 8-1(1), 256-3 and M1575 were phylogenetically related to the type strains of , , , , , , and , sharing 95.6-100 % 16S rRNA gene sequence similarities, less than 91.6 % gene sequence similarities, less than 98.2 % gene sequence similarities and less than 89.4 % ANI values. Based upon the data of polyphasic characterisation obtained in the present study, nine novel species, sp. nov., sp. nov., sp. nov., sp. nov., sp. nov., sp. nov., sp. nov., sp. nov. and sp. nov., are proposed and the type strains are 54-2 (=NCIMB 15154=CCM 8896=KCTC 21120=LMG 31058), 54-5 (=NCIMB 15151=CCM 8894), 33-7 (=NCIMB 15153=CCM 8936=KCTC 21118=LMG 31166), 116-2 (=NCIMB 15158=CCM 8899=KCTC 21124=LMG 31051), 184-8 (=NCIMB 15152=CCM 8895=KCTC 21131=LMG 31050), 204-8 (=NCIMB 15159=CCM 8900=KCTC 21133=LMG 31054), 8-1(1) (=NCIMB 15156=CCM 8898=KCTC 21115=LMG 31047), 256-3 (=NCIMB 15160=CCM 8901=LMG 31048) and M1575 (=NCIMB 15149=CCM 8892=LMG 31045), respectively.
从中国传统泡菜和酸奶中分离出 14 株革兰氏阳性细菌。采用多相分类方法对这些菌株进行了鉴定，包括 16S rRNA 基因序列分析、 基因序列分析、 基因序列分析、DNA G+C 含量测定、平均核苷酸同一性测定、脂肪酸甲酯分析和表型特征分析。数据表明，这 14 株菌代表了 9 个新种，属于 属，菌株 54-2、54-5、33-7、116-2、184-8、204-8、8-1(1)、256-3 和 M1575 被指定为模式菌株。菌株 54-2 与 和 的模式菌株亲缘关系最密切，16S rRNA 基因序列相似度为 96.5%和 91.6%， 基因序列相似度小于 74.6%， 基因序列相似度小于 81.6%，ANI 值小于 72.5%。菌株 54-5 与 和 的模式菌株亲缘关系最密切，16S rRNA 基因序列相似度为 99.1%和 97.3%， 基因序列相似度小于 83.1%， 基因序列相似度小于 93.1%，ANI 值小于 79.9%。菌株 33-7、116-2、184-8、204-8、8-1(1)、256-3 和 M1575 与 、 、 、 、 、 和 的模式菌株亲缘关系最密切，16S rRNA 基因序列相似度为 95.6%-100%， 基因序列相似度小于 91.6%， 基因序列相似度小于 98.2%，ANI 值小于 89.4%。基于本研究多相特征分析的数据，提出了 9 个新种，即 sp. nov.、 sp. nov.、 sp. nov.、 sp. nov.、 sp. nov.、 sp. nov.、 sp. nov.、 sp. nov.和 sp. nov.，模式菌株分别为 54-2(=NCIMB 15154=CCM 8896=KCTC 21120=LMG 31058)、54-5(=NCIMB 15151=CCM 8894)、33-7(=NCIMB 15153=CCM 8936=KCTC 21118=LMG 31166)、116-2(=NCIMB 15158=CCM 8899=KCTC 21124=LMG 31051)、184-8(=NCIMB 15152=CCM 8895=KCTC 21131=LMG 31050)、204-8(=NCIMB 15159=CCM 8900=KCTC 21133=LMG 31054)、8-1(1)(=NCIMB 15156=CCM 8898=KCTC 21115=LMG 31047)、256-3(=NCIMB 15160=CCM 8901=LMG 31048)和 M1575(=NCIMB 15149=CCM 8892=LMG 31045)。
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