Bioresource Collection and Research Center (BCRC), Food Industry Research and Development Institute. 331 Shih-Pin Rd, Hsinchu 30062, Taiwan, ROC.
Biological Resource Center (NBRC), National Institute of Technology and Evaluation (NITE), 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
Int J Syst Evol Microbiol. 2020 May;70(5):2972-2980. doi: 10.1099/ijsem.0.003522.
Four Gram-stain-positive strains, R7, R11, R19 and R27, were isolated from suan-tsai, a traditional fermented mustard green product of Taiwan. Cells were rod-shaped, non-motile, non-haemolytic, asporogenous, facultatively anaerobic, heterofermentative, and did not exhibit catalase and oxidase activities. Comparative analyses of 16S rRNA, and gene sequences demonstrated that these novel strains were members of the genus . 16S rRNA and the concatenated and gene sequence similarities between strains R7 and R11, and strains R19 and R27 were very high (>99.8 % similarity), respectively. On the basis of 16S rRNA gene sequence similarities, the type strains of (98.5 %), (98.5 %), (98.1 %) and (98.1 %) were the closest neighbours to strains R7 and R11, and the type strains of (98.9 %), (98.4 %), (98.4 %), (98.4 %) and (98.0 %) were the closest neighbours to strains R19 and R27, respectively. The average nucleotide identity values of R7 and R19 with the closely related type strains were 78.9-80.1% and 75.7-80.5 %, respectively. The digital DNA-DNA hybridization values were 22.8-23.6% and 21.0-23.1 %, respectively. Phenotypic and genotypic test results demonstrated that these strains represent two novel species of the genus , for which the name sp. nov. (R7=BCRC 81127=NBRC 113530) and sp. nov. (R19=BCRC 81129=NBRC 113532) are proposed.
从台湾传统发酵芥菜产品酸菜中分离到 4 株革兰氏阳性菌株 R7、R11、R19 和 R27。细胞呈杆状，不运动，不溶血，不产芽孢，兼性厌氧，异型发酵，无过氧化氢酶和氧化酶活性。16S rRNA 和 基因序列的比较分析表明，这些新菌株是 属的成员。R7 和 R11 株之间以及 R19 和 R27 株之间的 16S rRNA 和串联 和 基因序列相似性非常高（>99.8%相似）。基于 16S rRNA 基因序列相似性，（98.5%）、（98.5%）、（98.1%）和 （98.1%）的模式菌株与 R7 和 R11 株最为接近，而 R19 和 R27 株的模式菌株与 （98.9%）、（98.4%）、（98.4%）、（98.4%）和 （98.0%）最为接近。R7 和 R19 与密切相关的模式株的平均核苷酸同一性值分别为 78.9-80.1%和 75.7-80.5%。数字 DNA-DNA 杂交值分别为 22.8-23.6%和 21.0-23.1%。表型和基因型测试结果表明，这些菌株代表 属的两个新种，分别命名为 sp. nov.（R7=BCRC 81127=NBRC 113530）和 sp. nov.（R19=BCRC 81129=NBRC 113532）。
