Department of Chemistry, Faculty of Science, Shahid Rajaee Teacher Training University, Lavizan, P.O. Box 1678815811, Tehran, Iran.
Department of Health, Safety and Environment, School of Public Health and Safety, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Mater Sci Eng C Mater Biol Appl. 2019 Jun;99:121-128. doi: 10.1016/j.msec.2019.01.102. Epub 2019 Jan 24.
Developing of cheap, sensitive and stable sensors plays a significant role in pharmaceutical and clinical applications. Considering the effective role of Klonopin (KNP) in the treatment of epilepsy, KNP quantification in its production process for dose adjustments and checking the purity and also after its usage by patents for bioavailability testing and effectiveness assay is vital. In present work, an efficient electrochemical sensor based on poly melamine and multiwalled carbon nanotubes nanocomposite (PMela/CNTs) was constructed which displayed effective electrochemical response toward KNP. Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and square-wave voltammetry (SWV) experiments were applied for performance evaluation of the PMela/CNTs modified electrode and electrochemical redox behavior of KNP. Distinguish synergetic effect was observed between CNTs and poly melamine in response to KNP electrochemical redox reaction. A linear detection range of 0.05 to 10 μM with the detection limits of 63 nM was achieved for KNP analysis. The practical application of the PMela/CNTs modified electrode revealed satisfactory results for quantification of KNP in biological fluids.
开发廉价、灵敏、稳定的传感器在药物和临床应用中起着重要作用。鉴于 Klonopin（KNP）在治疗癫痫方面的有效作用，在其生产过程中对其进行定量分析以调整剂量、检查纯度，以及在专利中用于生物利用度测试和效果测定后对其进行定量分析，这是至关重要的。在本工作中，构建了一种基于三聚氰胺和多壁碳纳米管纳米复合材料（PMela/CNTs）的高效电化学传感器，该传感器对 KNP 表现出有效的电化学响应。电化学阻抗谱（EIS）、循环伏安法（CV）和方波伏安法（SWV）实验被应用于 PMela/CNTs 修饰电极的性能评估和 KNP 的电化学氧化还原行为。观察到 CNTs 和三聚氰胺之间在响应 KNP 电化学氧化还原反应时存在协同效应。实现了对 KNP 分析的 0.05 至 10 μM 的线性检测范围和 63 nM 的检测限。PMela/CNTs 修饰电极的实际应用为生物流体中 KNP 的定量分析提供了令人满意的结果。
