基于纳米晶纤维素修饰量子点的酪氨酸酶生物传感器用于苯酚测定。
Nanocrystalline cellulose decorated quantum dots based tyrosinase biosensor for phenol determination.
机构信息
Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia.
Department of Chemistry, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia; Institute of Advanced Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor D.E., Malaysia.
出版信息
Mater Sci Eng C Mater Biol Appl. 2019 Jun;99:37-46. doi: 10.1016/j.msec.2019.01.082. Epub 2019 Jan 21.
Novel biosensor architecture based on nanocrystalline cellulose (NCC)/CdS quantum dots (QDs) nanocomposite was developed for phenol determination. This nanocomposite was prepared with slight modification of nanocrystalline cellulose (NCC) with cationic surfactant of cetyltriammonium bromide (CTAB) and further decorated with 3-mercaptopropionic acid (3-MPA) capped CdS QDs. The nanocomposite material was then employed as scaffold for immobilization of tyrosinase enzyme (Tyr). The electrocatalytic response of Tyr/CTAB-NCC/QDs nanocomposite towards phenol was evaluated using differential pulse voltammetry (DPV). The current response obtained is proportional to the concentration of phenol which attributed to the reduction of o-quinone produced at the surface of the modified electrode. Under the optimal conditions, the biosensor exhibits good linearity towards phenol in the concentration range of 5-40 μM (R = 0.9904) with sensitivity and limit of detection (LOD) of 0.078 μA/μM and 0.082 μM, respectively.
基于纳米纤维素 (NCC)/CdS 量子点 (QDs) 纳米复合材料的新型生物传感器架构已被开发用于苯酚测定。该纳米复合材料通过用阳离子表面活性剂十六烷基三甲基溴化铵 (CTAB) 轻微修饰纳米纤维素 (NCC) 并进一步用 3-巯基丙酸 (3-MPA) 封端的 CdS QDs 进行修饰而制备。然后，将纳米复合材料用作固定化酪氨酸酶 (Tyr) 的支架。使用差分脉冲伏安法 (DPV) 评估 Tyr/CTAB-NCC/QDs 纳米复合材料对苯酚的电催化响应。获得的电流响应与苯酚的浓度成正比，这归因于修饰电极表面上产生的邻苯醌的还原。在最佳条件下，该生物传感器对 5-40 μM 范围内的苯酚表现出良好的线性关系（R=0.9904），灵敏度和检测限 (LOD) 分别为 0.078 μA/μM 和 0.082 μM。
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