Instituto de Investigaciones Biológicas del Trópico (IIBT), Facultad de Medicina Veterinaria y Zootecnia, Doctorado en Medicina Tropical, Universidad de Córdoba, Carrera 6 No 76-103., Monteria, Córdoba, Colombia.
Instituto de Investigaciones Biológicas del Trópico (IIBT), Programa Regencia en Farmacia, Facultad de Ciencias de la Salud, Doctorado en Medicina Tropical, Universidad de Córdoba, Monteria, Colombia.
Ann Clin Microbiol Antimicrob. 2019 Mar 19;18(1):11. doi: 10.1186/s12941-019-0308-y.
Bats are an important ecological group within ecosystems. The rabies virus is a Lyssavirus, and haematophagous bats are the principal reservoir; however, the virus has also been detected in non-haematophagous bats. The objective was to determine the rabies virus in non-haematophagous bats in the Colombian Caribbean region.
In 2017, a cross-sectional study was carried out with a base-risk sampling in twelve geographic zones of the Colombian Caribbean area that included the main ecosystems of two departments. 286 bats were captured, which were euthanized with a pharmacological treatment following the ethical protocols of animal experimentation. The taxonomic identification was done with dichotomous keys. The necropsy was carried out at the capture site, and brain samples were kept in liquid nitrogen. The extraction of the RNA was carried out from the frozen brains with Trizol™; a fragment of 914 bp of the glycoprotein G of the rabies virus was amplified with RT-PCR. The amplicons were sequenced with the Sanger method.
Twenty-three genera of bats were identified, and, in two frugivorous, Artibeus lituratus and Artibeus planirostris, amplicons were obtained and sequenced as the rabies virus.
This is the first evidence of natural infection of the rabies virus in frugivorous bats in the Colombian Caribbean area; this result is important for the surveillance and control of rabies.
蝙蝠是生态系统中一个重要的生态群体。狂犬病病毒是一种 Lyssavirus，吸血蝙蝠是主要的宿主；然而，该病毒也已在非吸血蝙蝠中检测到。目的是确定哥伦比亚加勒比地区非吸血蝙蝠中的狂犬病病毒。
2017 年，在哥伦比亚加勒比地区的 12 个地理区域进行了一项横断面研究，该研究采用基础风险抽样，包括两个部门的两个主要生态系统。共捕获了 286 只蝙蝠，这些蝙蝠在遵循动物实验伦理协议的情况下用药物治疗安乐死。通过二项式分类法进行分类鉴定。在捕获现场进行尸检，并将脑组织保存在液氮中。用 Trizol™从冷冻脑组织中提取 RNA；用 RT-PCR 扩增狂犬病病毒糖蛋白 G 的 914bp 片段。用 Sanger 法对扩增子进行测序。
鉴定出了 23 种蝙蝠属，在两种食果蝙蝠（Artibeus lituratus 和 Artibeus planirostris）中获得并测序了狂犬病病毒的扩增子。
这是哥伦比亚加勒比地区首次在食果蝙蝠中自然感染狂犬病病毒的证据；这一结果对狂犬病的监测和控制非常重要。
