Key Laboratory of Genetics and Fruit Development, College of Horticulture, Nanjing Agricultural University, Nanjing 210095, PR China.
Laboratory of Plant Healthcare and Diagnostics, P.G. Department of Biotechnology and Microbiology, Karnataka University, Dharwad, India.
Gene. 2019 Jun 20;702:27-35. doi: 10.1016/j.gene.2019.03.025. Epub 2019 Mar 16.
The protein phosphatase 2C (PP2C) gene family is one of the momentous and conserved plant-specific gene families, known to participate in cellular processes via reversible protein phosphorylation and regulates signal transduction in eukaryotic organisms. Recently, PP2Cs were identified in Arabidopsis and maize, however, the whole-genome analysis of PP2C in strawberry has not yet been reported. In the current research, we found 62 PP2C-encoding genes in total from the strawberry genome. Further, the phylogenetic analysis categorized FvPP2C genes into twelve subgroups with significant structural conservation based on conserved domain and amino acid sequence. Moreover, we observed a strong signature of purifying selection between the comparison of orthologous gene pairs of strawberry and Arabidopsis. The comparison of RNA-sequence (RNA-seq) data published on various vegetative and reproductive tissues of strawberry plant suggested the significant role of FvPP2C genes in organ development. The qRT-PCR validation of thirty FvPP2C genes indicated their critical tolerance-related role under abiotic stress stimuli in strawberry. Finally, the subcellular localization of FvPP2C51 gene proves that it resides and stimulates its function in the nucleus. Our findings provide an overview of the identification of strawberry FvPP2C gene family and demonstrate their critical role in tissue-specific response and abiotic stress-tolerance, thereby, intimating their significance in the strawberry molecular breeding for the resistant cultivars.
蛋白磷酸酶 2C（PP2C）基因家族是一个重要且保守的植物特异性基因家族，通过可逆的蛋白质磷酸化参与细胞过程，并调节真核生物中的信号转导。最近，在拟南芥和玉米中鉴定出了 PP2C，但草莓全基因组分析尚未报道。在当前的研究中，我们从草莓基因组中总共发现了 62 个编码 PP2C 的基因。进一步的系统发育分析根据保守结构域和氨基酸序列将 FvPP2C 基因分为十二个亚组，具有显著的结构保守性。此外，我们观察到草莓和拟南芥同源基因对之间存在强烈的纯化选择信号。对草莓不同营养和生殖组织的 RNA-seq 数据的比较表明，FvPP2C 基因在器官发育中起着重要作用。对 30 个 FvPP2C 基因的 qRT-PCR 验证表明，它们在草莓非生物胁迫刺激下具有重要的耐逆相关作用。最后，FvPP2C51 基因的亚细胞定位证明它位于细胞核内并刺激其功能。我们的研究结果提供了草莓 FvPP2C 基因家族的鉴定概述，并证明了它们在组织特异性反应和非生物胁迫耐受性中的关键作用，从而暗示它们在草莓分子育种中对抗性品种的重要性。
