Sun Bo, Tian Yu-Xiao, Chen Qing, Zhang Yong, Luo Ya, Wang Yan, Li Meng-Yao, Gong Rong-Gao, Wang Xiao-Rong, Zhang Fen, Tang Hao-Ru
College of Horticulture, Sichuan Agricultural University, Chengdu 611130, People's Republic of China.
Institute of Pomology and Olericulture, Sichuan Agricultural University, Chengdu 611130, People's Republic of China.
R Soc Open Sci. 2019 Feb 20;6(2):182054. doi: 10.1098/rsos.182054. eCollection 2019 Feb.
The composition and content of glucosinolates were investigated in the edible parts (petioles, peel and flesh) of tuber mustard, bamboo shoots mustard and baby mustard by high-performance liquid chromatography to reveal the association between the different cooking methods and their glucosinolate profiles. Eight glucosinolates were identified from tuber mustard and baby mustard, including three aliphatic glucosinolates, four indole glucosinolates and one aromatic glucosinolate. Only six of the eight glucosinolates were detected in bamboo shoots mustard. The results show that the distribution and content of glucosinolates varied widely among the different tissues and species. The highest contents of glucosinolates in tuber mustard, bamboo shoots mustard and baby mustard were found in flesh, petioles and peel, respectively. The content of total glucosinolates ranged from 5.21 µmol g dry weight in bamboo shoots mustard flesh to 25.64 µmol g dry weight in baby mustard peel. Aliphatic glucosinolates were predominant in the three stem mustards, followed by indole and aromatic glucosinolates. Sinigrin was the predominant glucosinolate in the three stem mustards. Sinigrin content in tuber mustard was slightly higher than that in baby mustard and much higher than that in bamboo shoots mustard, suggesting that the pungent-tasting stem mustards contained more sinigrin. In addition, a principal components analysis showed that bamboo shoots mustard was distinguishable from the other two stem mustards. A variance analysis indicated that the glucosinolates were primarily influenced by a species × tissue interaction. The correlations among glucosinolates were also analysed.
采用高效液相色谱法对榨菜、笋子芥和儿菜的可食用部分(叶柄、外皮和肉质)中的硫代葡萄糖苷的组成和含量进行了研究,以揭示不同烹饪方法与其硫代葡萄糖苷谱之间的关联。从榨菜和儿菜中鉴定出8种硫代葡萄糖苷,包括3种脂肪族硫代葡萄糖苷、4种吲哚硫代葡萄糖苷和1种芳香族硫代葡萄糖苷。在笋子芥中仅检测到8种硫代葡萄糖苷中的6种。结果表明,硫代葡萄糖苷的分布和含量在不同组织和品种间差异很大。榨菜、笋子芥和儿菜中硫代葡萄糖苷含量最高的部位分别是肉质、叶柄和外皮。总硫代葡萄糖苷含量范围从笋子芥肉质中的5.21 μmol/g干重到儿菜外皮中的25.64 μmol/g干重。脂肪族硫代葡萄糖苷在这三种茎用芥菜中占主导地位,其次是吲哚和芳香族硫代葡萄糖苷。黑芥子硫苷酸钾是这三种茎用芥菜中的主要硫代葡萄糖苷。榨菜中的黑芥子硫苷酸钾含量略高于儿菜,远高于笋子芥,这表明味道辛辣的茎用芥菜含有更多的黑芥子硫苷酸钾。此外,主成分分析表明笋子芥与其他两种茎用芥菜有区别。方差分析表明,硫代葡萄糖苷主要受品种×组织相互作用的影响。还分析了硫代葡萄糖苷之间的相关性。