Marquina Clara, Mousa Aya, Belski Regina, Banaharis Harry, Naderpoor Negar, de Courten Barbora
Monash Centre for Health Research and Implementation, School of Public Health and Preventive Medicine, Monash University, Melbourne 3004, Australia.
Faculty of Health, Arts and Design, Swinburne University of Technology, Melbourne 3122, Australia.
J Clin Med. 2019 Mar 19;8(3):382. doi: 10.3390/jcm8030382.
Lower copy number variations (CNVs) in the salivary amylase gene () have been associated with obesity and insulin resistance; however, the relationship between and cardiometabolic risk has not been fully elucidated. Using gold-standard measures, we aimed to examine whether CNVs are associated with cardiometabolic risk factors in an overweight or obese, otherwise healthy population. Fifty-seven adults (58% male) aged 31.17 ± 8.44 years with a body mass index (BMI) ≥25 kg/m² were included in the study. We measured CNVs (qPCR); anthropometry (BMI; body composition by dual-energy X-ray absorptiometry); cardiovascular parameters (blood pressure, serum lipids by ELISA); insulin sensitivity (hyperinsulinaemic⁻euglycaemic clamp), insulin secretion (intravenous glucose tolerance test), and serum inflammation markers (multiplex assays). Based on previous studies and median values, participants were divided into low (≤4) and high (>4) CNV groups. Low carriers ( = 29) had a higher fat mass (40.76 ± 12.11 versus 33.33 ± 8.50 kg, = 0.009) and LDL-cholesterol (3.27 ± 0.80 versus 2.87 ± 0.69 mmol/L, = 0.038), and higher serum levels of interleukin [IL]-6, IL-1β, tumour necrosis factor-alpha and monocyte chemoattractant protein-1 (MCP-1) (all < 0.05) compared with high carriers ( = 28), but there were no differences in glycaemic measures, including insulin sensitivity or secretion (all > 0.1). Except for MCP-1, the results remained significant in multivariable models adjusted for age, sex, and fat mass (all < 0.05). Our findings suggest that low CNVs are associated with increased cardiovascular disease risk and inflammation, but not glucose metabolism, in overweight or obese adults.
唾液淀粉酶基因（）中较低的拷贝数变异（CNV）与肥胖和胰岛素抵抗相关；然而，该基因与心脏代谢风险之间的关系尚未完全阐明。我们采用金标准测量方法，旨在研究在超重或肥胖但其他方面健康的人群中，唾液淀粉酶基因CNV是否与心脏代谢风险因素相关。研究纳入了57名年龄为31.17±8.44岁、体重指数（BMI）≥25kg/m²的成年人（58%为男性）。我们测量了唾液淀粉酶基因CNV（定量聚合酶链反应）；人体测量指标（BMI；通过双能X线吸收法测量身体成分）；心血管参数（血压、通过酶联免疫吸附测定法测量血脂）；胰岛素敏感性（高胰岛素-正葡萄糖钳夹技术）、胰岛素分泌（静脉葡萄糖耐量试验）以及血清炎症标志物（多重分析）。根据先前研究和中位数，参与者被分为低（≤4）和高（>4）唾液淀粉酶基因CNV组。低唾液淀粉酶基因携带者（n = 29）相比于高唾液淀粉酶基因携带者（n = 28），具有更高的脂肪量（40.76±12.11对33.33±8.50kg，P = 0.009）和低密度脂蛋白胆固醇（3.27±0.80对2.87±0.69mmol/L，P = 0.038）以及更高的血清白细胞介素[IL]-6、IL-1β、肿瘤坏死因子-α和单核细胞趋化蛋白-1（MCP-1）水平（均P < 0.05），但在血糖指标方面，包括胰岛素敏感性或分泌，均无差异（均P > 0.1）。除MCP-1外，在调整了年龄、性别和脂肪量的多变量模型中，结果仍然显著（均P < 0.05）。我们的研究结果表明，在超重或肥胖成年人中，低唾液淀粉酶基因CNV与心血管疾病风险增加和炎症相关，但与糖代谢无关。
