糖酵解产生的乳酸促成了葡萄糖诱导的金黄色葡萄球菌聚集。
Lactic Acid Produced by Glycolysis Contributed to Staphylococcus aureus Aggregation Induced by Glucose.
作者信息
Luo Zhen, Chen Meilin, Chen Ti, She Pengfei, Wu Yong
机构信息
Department of Clinical Laboratory, The Third Xiangya Hospital of Central South University, Changsha, China.
Xiangya School of Medicine, Central South University, Changsha, China.
出版信息
Curr Microbiol. 2019 May;76(5):607-612. doi: 10.1007/s00284-019-01666-z. Epub 2019 Mar 20.
High concentration of glucose induces Staphylococcus aureus (S. aureus) aggregation, but the mechanism of this is still unclear. In this study, the aggregation of S. aureus strains was induced by high concentration of glucose (>7.8 mM), and which was dose- and time-dependent. In addition, the large amount of lactate acid produced during S. aureus aggregation, induced by glucose, resulted in decreased pH value. Lactic acid, the end product of glycolysis, could quickly induce S. aureus aggregation. Except for lactic acid, acetic acid and HCl also induced S. aureus aggregation. In addition, the aggregation of S. aureus strains induced by glucose or lactic acid was completely inhibited in Tris-HCl buffer (pH 7.5), and inhibition of glycolysis by 2-deoxyglucose significantly decreased S. aureus aggregation. The aggregation induced by glucose was dispersed by periodate and proteinase K. In summary, lactate acid produced by glycolysis contributed to S. aureus aggregation induced by high concentration of glucose.
高浓度葡萄糖可诱导金黄色葡萄球菌聚集，但其机制尚不清楚。在本研究中，高浓度葡萄糖（>7.8 mM）可诱导金黄色葡萄球菌菌株聚集，且呈剂量和时间依赖性。此外，葡萄糖诱导金黄色葡萄球菌聚集过程中产生的大量乳酸导致pH值降低。糖酵解的终产物乳酸可迅速诱导金黄色葡萄球菌聚集。除乳酸外，乙酸和盐酸也可诱导金黄色葡萄球菌聚集。此外，在Tris-HCl缓冲液（pH 7.5）中，葡萄糖或乳酸诱导的金黄色葡萄球菌菌株聚集被完全抑制，2-脱氧葡萄糖对糖酵解的抑制显著降低了金黄色葡萄球菌的聚集。葡萄糖诱导的聚集可被高碘酸盐和蛋白酶K分散。综上所述，糖酵解产生的乳酸促成了高浓度葡萄糖诱导的金黄色葡萄球菌聚集。
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