Popova Anastasia V, Shneider Mikhail M, Myakinina Vera P, Bannov Vasily A, Edelstein Mikhail V, Rubalskii Evgenii O, Aleshkin Andrey V, Fursova Nadezhda K, Volozhantsev Nikolay V
Moscow Institute of Physics and Technology (State University), Dolgoprudny, Moscow Region, Russia.
Institute of Antimicrobial Chemotherapy, Smolensk State Medical University, Smolensk, Russia.
Arch Virol. 2019 May;164(5):1493-1497. doi: 10.1007/s00705-019-04208-x. Epub 2019 Mar 20.
In the present study, we investigate the biological properties and genomic organization of virulent bacteriophage AM24, which specifically infects multidrug-resistant clinical Acinetobacter baumannii strains with a K9 capsular polysaccharide structure. The phage was identified as a member of the family Myoviridae by transmission electron microscopy. The AM24 linear double-stranded DNA genome of 97,177 bp contains 167 open reading frames. Putative functions were assigned for products of 40 predicted genes, including proteins involved in nucleotide metabolism and DNA replication, packaging of DNA into the capsid, phage assembly and structural proteins, and bacterial cell lysis. The gene encoding the tailspike, which possesses depolymerase activity towards the corresponding capsular polysaccharides, is situated in the phage genome outside of the structural module, upstream of the genes responsible for packaging of DNA into the capsid. The data on characterization of depolymerase-carrying phage AM24 contributes to our knowledge of the diversity of viruses infecting different capsular types of A. baumannii.
在本研究中，我们调查了烈性噬菌体AM24的生物学特性和基因组组织，该噬菌体特异性感染具有K9荚膜多糖结构的多重耐药临床鲍曼不动杆菌菌株。通过透射电子显微镜鉴定该噬菌体为肌尾噬菌体科的成员。AM24的线性双链DNA基因组为97,177 bp，包含167个开放阅读框。为40个预测基因的产物指定了推定功能，包括参与核苷酸代谢和DNA复制、将DNA包装到衣壳中、噬菌体组装和结构蛋白以及细菌细胞裂解的蛋白质。编码对相应荚膜多糖具有解聚酶活性的尾刺的基因位于噬菌体基因组结构模块之外，在负责将DNA包装到衣壳中的基因上游。携带解聚酶的噬菌体AM24的表征数据有助于我们了解感染不同荚膜类型鲍曼不动杆菌的病毒的多样性。
