Bunge 水提取物对高脂饮食和链脲佐菌素诱导的 2 型糖尿病小鼠炎症细胞因子释放和肠道微生物群的有益作用。

Beneficial Effects of Bunge Water Extract on Inflammatory Cytokines Release and Gut Microbiota in High-Fat Diet and Streptozotocin-Induced Type 2 Diabetic Mice.

机构信息

Beijing Advanced Innovation Center for Food Nutrition and Human Health, College of Food Science & Nutritional Engineering, China Agricultural University, Beijing 100083, China.

Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, Ministry of Education, China Agricultural University, Beijing 100083, China.

出版信息

Nutrients. 2019 Mar 20;11(3):670. doi: 10.3390/nu11030670.

Bunge (PDB), a perennial herb, has been used as a traditional Chinese medicine in the therapy of many diseases. The aim of the current study was to investigate the effect of PDB water extract on systemic inflammation and gut microbiota in type 2 diabetic (T2D) mice induced by high-fat diet (HFD) and streptozotocin (STZ) injection. C57BL/6J mice were randomly divided into a normal diet (ND) group, T2D group, and PDB group (diabetic mice treated with PDB water extract at a dose of 400 mg/kg body weight). Results showed that PDB significantly decreased the levels of lipopolysaccharide (LPS) and pro-inflammatory cytokines in serum. Further investigation showed that PDB significantly reduced the ratio of / and the relative abundance of in fecal samples of diabetic mice. In addition, PDB notably alleviated intestinal inflammation as evidenced by decreased expression of toll-like receptor 4 (TLR4), myeloid differentiation factor 88 (MyD88), nuclear factor-κB (NF-κB), and inflammatory cytokines. PDB also reversed the decreased expression of intestinal mucosal tight junction proteins including Claudin3, ZO-1, and Occludin. Meanwhile, the levels of fecal acetic acid and butyric acid and their specific receptors including G-protein-coupled receptor (GPR) 41 and 43 expression in the colon were also increased after PDB treatment. Our results indicated that PDB might serve as a potential functional ingredient against diabetes and related inflammation.

荜茇（PDB），一种多年生草本植物，已被用作治疗多种疾病的传统中药。本研究旨在探讨 PDB 水提取物对高脂肪饮食（HFD）和链脲佐菌素（STZ）注射诱导的 2 型糖尿病（T2D）小鼠全身炎症和肠道微生物群的影响。C57BL/6J 小鼠随机分为正常饮食（ND）组、T2D 组和 PDB 组（糖尿病小鼠用 PDB 水提取物以 400mg/kg 体重的剂量治疗）。结果表明，PDB 显著降低了血清中脂多糖（LPS）和促炎细胞因子的水平。进一步的研究表明，PDB 显著降低了糖尿病小鼠粪便中 / 的比值和的相对丰度。此外，PDB 明显减轻了肠道炎症，表现为 TLR4、MyD88、NF-κB 和炎症细胞因子的表达降低。PDB 还逆转了肠道黏膜紧密连接蛋白 Claudin3、ZO-1 和 Occludin 的表达降低。同时，PDB 处理后，粪便中乙酸和丁酸的水平及其在结肠中的特定受体，包括 G 蛋白偶联受体（GPR）41 和 43 的表达也增加了。我们的结果表明，PDB 可能是一种潜在的功能性成分，可用于治疗糖尿病及其相关炎症。