Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Pharmaceutical Sciences, Jiangnan University, Wuxi, 214122, PR China.
Key Laboratory of Carbohydrate Chemistry and Biotechnology, Ministry of Education, School of Pharmaceutical Sciences, Jiangnan University, Wuxi, 214122, PR China; National Engineering Laboratory for Cereal Fermentation Technology, School of Biotechnology, Jiangnan University, Wuxi, 214122, PR China.
J Ethnopharmacol. 2019 Jul 15;239:111854. doi: 10.1016/j.jep.2019.111854. Epub 2019 Apr 5.
In Chinese folk medicine, the flower of Edgeworthia gardneri (Wall.) Meisn. is used to treat various metabolic diseases, such as hyperglycemia, hypertension, and hyperlipidemia.
This study aimed to explore the antidiabetes potential of the flower of E. gardneri and investigate whether it can benefit the entire gut bacteria community.
Chemical constituents of the extract were analyzed by UHPLC-Q Exactive Mass Spectrometer (UHPLC-QE-MS). The antidiabetes effect of the water extract (WAE) of the flower of E. gardneri was evaluated in diabetic mice induced by high-fat diet (HFD) and streptozotocin (STZ) (six groups, n = 8) daily at doses of 1, 2, and 3 g/kg for 4 weeks. The gut microbiota was analyzed using high-throughput 16S rRNA gene sequencing. Short-chain fatty acids (SCFAs) in the fecal were also investigated.
UHPLC-QE-MS analysis identified 29 compounds, including five alkaloids, six coumarins, four flavonoids, 11 organic acids, and three additional compounds, in the WAE. Results showed that the high dose of WAE considerably decreased the blood glucose level by 30.0%. Furthermore, E. gardneri significantly ameliorated insulin resistance and lipid metabolism dysfunction and repaired islet, hepatic, and white fat and colon histology in diabetic mice. Diabetic mice treated with WAE showed apparent changes in the structure and composition of the gut microbiota. WAE reversed the changes in Clostridiales, Lachnospiraceae, S24-7, Rikenellaceae, and Dorea in diabetic mice. The correlation analysis indicated that key OTUs were related to diabetes indices. The amounts of SCFAs, including acetic, propionic, and valeric acids, were significantly high in WAE-treated diabetic groups.
E. gardneri treatment improved the glucose metabolism and reshaped the unbalanced gut microbiota of diabetic mice. Our study provides evidence for application of E. gardneri to treatment of diabetes mellitus.
在中国民间医学中，蜡梅科蜡梅属植物蜡梅花（Wall.）Meisn. 被用于治疗各种代谢疾病，如高血糖、高血压和高血脂。
本研究旨在探索蜡梅花的降血糖潜力，并研究其是否有益于整个肠道细菌群落。
采用 UHPLC-Q Exactive Mass Spectrometer（UHPLC-QE-MS）分析提取物的化学成分。用高脂饮食（HFD）和链脲佐菌素（STZ）诱导糖尿病小鼠（六组，n=8），每天灌胃蜡梅花水提物（WAE）1、2 和 3 g/kg，连续 4 周，评价其降血糖作用。采用高通量 16S rRNA 基因测序分析肠道微生物群。还研究了粪便中的短链脂肪酸（SCFAs）。
UHPLC-QE-MS 分析鉴定了 WAE 中的 29 种化合物，包括 5 种生物碱、6 种香豆素、4 种类黄酮、11 种有机酸和 3 种其他化合物。结果表明，高剂量的 WAE 可使血糖水平降低 30.0%。此外，蜡梅花显著改善了糖尿病小鼠的胰岛素抵抗和脂质代谢功能，并修复了糖尿病小鼠的胰岛、肝、白色脂肪和结肠组织学。用 WAE 处理的糖尿病小鼠的肠道微生物群结构和组成发生明显变化。WAE 逆转了糖尿病小鼠中梭状芽胞杆菌、lachnospiraceae、S24-7、Rikenellaceae 和 Dorea 的变化。相关分析表明，关键 OTUs 与糖尿病指数相关。用 WAE 处理的糖尿病组中，包括乙酸、丙酸和缬草酸在内的 SCFAs 含量显著升高。
蜡梅花治疗改善了糖尿病小鼠的葡萄糖代谢，并重塑了其失衡的肠道微生物群。本研究为蜡梅花治疗糖尿病提供了依据。
