抗脂质和抗蛋白质单克隆抗体对肺炎支原体的生物学效应
Biological effects of anti-lipid and anti-protein monoclonal antibodies on Mycoplasma pneumoniae.
作者信息
Morrison-Plummer J, Leith D K, Baseman J B
出版信息
Infect Immun. 1986 Aug;53(2):398-403. doi: 10.1128/iai.53.2.398-403.1986.
Abstract
Monoclonal antibodies directed against Mycoplasma pneumoniae surface components were examined for their ability to block mycoplasma attachment to chicken erythrocytes. Purified preparations of antibodies which recognize the major mycoplasma ligand mediating cytadherence (protein P1, 165 kilodaltons) inhibited attachment by more than 85% of the control values. Monoclonal antibodies reactive with two other surface proteins of 110 and 32 kilodaltons also blocked attachment. Surprisingly, monoclonal antibodies specific for M. pneumoniae lipids (J. Morrison-Plummer, D. H. Jones, and J. B. Baseman, J. Immunol. Methods 64:165-178, 1983) enhanced mycoplasma-erythrocyte binding. All antibodies examined had no effect on thymidine incorporation by M. pneumoniae.
摘要
检测了针对肺炎支原体表面成分的单克隆抗体阻断支原体黏附鸡红细胞的能力。识别介导细胞黏附的主要支原体配体（165千道尔顿的蛋白P1）的纯化抗体制剂抑制黏附的程度超过对照值的85%。与另外两种110千道尔顿和32千道尔顿表面蛋白反应的单克隆抗体也能阻断黏附。令人惊讶的是，针对肺炎支原体脂质的单克隆抗体（J.莫里森 - 普拉默、D.H.琼斯和J.B.贝斯门，《免疫学方法杂志》64:165 - 178，1983）增强了支原体 - 红细胞结合。所检测的所有抗体对肺炎支原体的胸苷掺入均无影响。
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