长链非编码RNA SNHG7通过对miR-9的负调控促进骨折修复。
Long non-coding RNA SNHG7 promotes the fracture repair through negative modulation of miR-9.
作者信息
Chen Zhiqing, Liu Zhi, Shen Lin, Jiang Han
机构信息
Department of Osteology, Tianjin Third Central Hospital Tianjin 300170, China.
Tianjin Institute of Hepatobiliary Disease Tianjin 300170, China.
出版信息
Am J Transl Res. 2019 Feb 15;11(2):974-982. eCollection 2019.
Abstract
Fracture is the most common disease in the orthopedics. Long non-coding small nucleolar RNA host gene 7 (SNHG7) has been confirmed to enhance cell proliferation and decrease cell apoptosis in many cancers. However, the role of SNHG7 in skeletal fracture remains largely to be elucidated. In the current study, we observed SNHG7 was down-regulated in femoral neck fracture tissues. In addition, SNHG7 knockdown inhibited proliferation and migration, induced apoptosis, reduced activity in osteoblast cells in vitro. Bioinformatics analysis revealed SNHG7 acts as a molecular sponge for miR-9 and MiR-9 directly targets with 3'-UTR of TGFBR2. Furthermore, SNHG7 knockdown repressed the TGF-β signaling pathway. Taken together, this study manifested SNHG7 promotes bone repair in femoral neck fracture, and may serve as a potential target for enhancing bone formation.
摘要
骨折是骨科最常见的疾病。长链非编码小核仁RNA宿主基因7（SNHG7）已被证实在许多癌症中可增强细胞增殖并减少细胞凋亡。然而，SNHG7在骨骼骨折中的作用仍有待进一步阐明。在本研究中，我们观察到SNHG7在股骨颈骨折组织中表达下调。此外，SNHG7基因敲低抑制了体外成骨细胞的增殖和迁移，诱导了细胞凋亡，降低了细胞活性。生物信息学分析显示，SNHG7作为miR-9的分子海绵，而miR-9直接靶向TGFBR2的3'-UTR。此外，SNHG7基因敲低抑制了TGF-β信号通路。综上所述，本研究表明SNHG7促进股骨颈骨折的骨修复，并可能成为促进骨形成的潜在靶点。
相似文献
1
Long non-coding RNA SNHG7 promotes the fracture repair through negative modulation of miR-9.长链非编码RNA SNHG7通过对miR-9的负调控促进骨折修复。
Am J Transl Res. 2019 Feb 15;11(2):974-982. eCollection 2019.
2
Long noncoding RNA SNHG7 promotes the progression and growth of glioblastoma via inhibition of miR-5095.长链非编码RNA SNHG7通过抑制miR-5095促进胶质母细胞瘤的进展和生长。
Biochem Biophys Res Commun. 2018 Feb 5;496(2):712-718. doi: 10.1016/j.bbrc.2018.01.109.
3
Long non-coding RNA SNHG7 promotes malignant melanoma progression through negative modulation of miR-9.长链非编码 RNA SNHG7 通过负向调控 miR-9 促进恶性黑色素瘤进展。
Histol Histopathol. 2020 Sep;35(9):973-981. doi: 10.14670/HH-18-225. Epub 2020 May 4.
4
lncRNA SNHG7 promotes cell proliferation in glioma by acting as a competing endogenous RNA and sponging miR-138-5p to regulate EZH2 expression.长链非编码RNA SNHG7通过作为竞争性内源性RNA并吸附miR-138-5p来调节EZH2表达，从而促进胶质瘤细胞增殖。
Oncol Lett. 2021 Jul;22(1):565. doi: 10.3892/ol.2021.12826. Epub 2021 May 29.
5
Long Noncoding RNA SNHG7 Promotes the Tumor Growth and Epithelial-to-Mesenchymal Transition via Regulation of miR-34a Signals in Osteosarcoma.长链非编码 RNA SNHG7 通过调节骨肉瘤中 miR-34a 信号促进肿瘤生长和上皮间质转化。
Cancer Biother Radiopharm. 2018 Nov;33(9):365-372. doi: 10.1089/cbr.2018.2503. Epub 2018 Jul 10.
6
Long non-coding RNA small nucleolar RNA host gene 7 is upregulated and promotes cell proliferation in thyroid cancer.长链非编码RNA小核仁RNA宿主基因7在甲状腺癌中上调并促进细胞增殖。
Oncol Lett. 2019 Nov;18(5):4726-4734. doi: 10.3892/ol.2019.10782. Epub 2019 Aug 27.
7
LncRNA SNHG7 contributes to tumorigenesis and progression in breast cancer by interacting with miR-34a through EMT initiation and the Notch-1 pathway.长链非编码 RNA SNHG7 通过 EMT 起始和 Notch-1 通路与 miR-34a 相互作用，促进乳腺癌的发生和发展。
Eur J Pharmacol. 2019 Aug 5;856:172407. doi: 10.1016/j.ejphar.2019.172407. Epub 2019 May 25.
8
Loss of lncRNA-SNHG7 Promotes the Suppression of Hepatic Stellate Cell Activation via miR-378a-3p and DVL2.lncRNA-SNHG7的缺失通过miR-378a-3p和DVL2促进肝星状细胞激活的抑制。
Mol Ther Nucleic Acids. 2019 Sep 6;17:235-244. doi: 10.1016/j.omtn.2019.05.026. Epub 2019 Jun 7.
9
Long non-coding RNA-SNHG7 acts as a target of miR-34a to increase GALNT7 level and regulate PI3K/Akt/mTOR pathway in colorectal cancer progression.长链非编码 RNA-SNHG7 作为 miR-34a 的靶标增加 GALNT7 水平并调节结直肠癌进展中的 PI3K/Akt/mTOR 通路。
J Hematol Oncol. 2018 Jul 3;11(1):89. doi: 10.1186/s13045-018-0632-2.
10
Long non-coding RNA SNHG7 inhibits NLRP3-dependent pyroptosis by targeting the miR-34a/SIRT1 axis in liver cancer.长链非编码RNA SNHG7通过靶向肝癌中的miR-34a/SIRT1轴抑制NLRP3依赖性细胞焦亡。
Oncol Lett. 2020 Jul;20(1):893-901. doi: 10.3892/ol.2020.11635. Epub 2020 May 18.
引用本文的文献
1
The Roles of Family in Osteoblast Differentiation.家庭在成骨细胞分化中的作用。
Genes (Basel). 2022 Dec 2;13(12):2268. doi: 10.3390/genes13122268.
2
[Regulation of long non-coding RNA in signal pathways related to osteogenic differentiation].[长链非编码RNA在成骨分化相关信号通路中的调控]
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2022 Apr 15;36(4):479-486. doi: 10.7507/1002-1892.202111098.
3
Oncogenic Roles of Small Nucleolar RNA Host Gene 7 (SNHG7) Long Noncoding RNA in Human Cancers and Potentials.小核仁RNA宿主基因7（SNHG7）长链非编码RNA在人类癌症中的致癌作用及潜力
Front Cell Dev Biol. 2022 Jan 17;9:809345. doi: 10.3389/fcell.2021.809345. eCollection 2021.
4
The Involvement of Long Non-Coding RNAs in Bone.长链非编码 RNA 在骨中的作用
Int J Mol Sci. 2021 Apr 10;22(8):3909. doi: 10.3390/ijms22083909.
5
Roles of the SNHG7/microRNA‑9‑5p/DPP4 ceRNA network in the growth and I resistance of thyroid carcinoma cells through PI3K/Akt activation.SNHG7/miR-9-5p/DPP4 竞争 RNA 网络通过激活 PI3K/Akt 在甲状腺癌细胞生长和碘抵抗中的作用。
Oncol Rep. 2021 Apr;45(4). doi: 10.3892/or.2021.7954. Epub 2021 Mar 2.
6
Long Non-Coding RNA SNHG7 Alleviates Oxygen and Glucose Deprivation/Reoxygenation-Induced Neuronal Injury by Modulating miR-9/SIRT1 Axis in PC12 Cells: Potential Role in Ischemic Stroke.长链非编码RNA SNHG7通过调节PC12细胞中的miR-9/SIRT1轴减轻氧糖剥夺/复氧诱导的神经元损伤：在缺血性卒中中的潜在作用
Neuropsychiatr Dis Treat. 2020 Nov 24;16:2837-2848. doi: 10.2147/NDT.S273421. eCollection 2020.
7
SNHG7 accelerates cell migration and invasion through regulating miR-34a-Snail-EMT axis in gastric cancer.SNHG7 通过调控 miR-34a-Snail-EMT 轴促进胃癌细胞迁移和侵袭。
Cell Cycle. 2020 Jan;19(1):142-152. doi: 10.1080/15384101.2019.1699753. Epub 2019 Dec 8.
本文引用的文献
1
LncRNA SNHG7 promotes the proliferation of esophageal cancer cells and inhibits its apoptosis.长链非编码 RNA SNHG7 促进食管癌细胞的增殖并抑制其凋亡。
Eur Rev Med Pharmacol Sci. 2018 May;22(9):2653-2661. doi: 10.26355/eurrev_201805_14961.
2
Knockdown of long non-coding RNA XIST suppresses nasopharyngeal carcinoma progression by activating miR-491-5p.敲低长非编码 RNA XIST 通过激活 miR-491-5p 抑制鼻咽癌进展。
J Cell Biochem. 2018 May;119(5):3936-3944. doi: 10.1002/jcb.26535. Epub 2018 Jan 22.
3
LncRNA SNHG7 promotes the proliferation and inhibits apoptosis of gastric cancer cells by repressing the P15 and P16 expression.长链非编码 RNA SNHG7 通过抑制 P15 和 P16 的表达促进胃癌细胞的增殖并抑制其凋亡。
Eur Rev Med Pharmacol Sci. 2017 Oct;21(20):4613-4622.
4
A comprehensive genome-wide analysis of long noncoding RNA expression profile in hepatocellular carcinoma.肝细胞癌中长链非编码RNA表达谱的全基因组综合分析。
Cancer Med. 2017 Dec;6(12):2932-2941. doi: 10.1002/cam4.1180. Epub 2017 Oct 18.
5
Targeting noncoding RNAs in disease.针对疾病中的非编码RNA
J Clin Invest. 2017 Mar 1;127(3):761-771. doi: 10.1172/JCI84424.
6
MiR-142-5p promotes bone repair by maintaining osteoblast activity.微小RNA-142-5p通过维持成骨细胞活性促进骨修复。
J Bone Miner Metab. 2017 May;35(3):255-264. doi: 10.1007/s00774-016-0757-8. Epub 2016 Apr 16.
7
TGFβ-2 signaling is essential for osteoblast migration and differentiation during fracture healing in medaka fish.转化生长因子β-2信号对于青鳉鱼骨折愈合过程中破骨细胞的迁移和分化至关重要。
Bone. 2016 May;86:68-78. doi: 10.1016/j.bone.2016.03.001. Epub 2016 Mar 4.
8
Long Noncoding RNA H19 Promotes Osteoblast Differentiation Via TGF-β1/Smad3/HDAC Signaling Pathway by Deriving miR-675.长链非编码 RNA H19 通过衍生 miR-675 促进 TGF-β1/Smad3/HDAC 信号通路促进成骨细胞分化。
Stem Cells. 2015 Dec;33(12):3481-92. doi: 10.1002/stem.2225. Epub 2015 Oct 23.
9
TGF-β superfamily, molecular signaling and biomimetic features for bone regeneration: historical perspectives and future applications.转化生长因子-β超家族、骨再生的分子信号传导及仿生特性：历史回顾与未来应用
Updates Surg. 2015 Sep;67(3):321-3. doi: 10.1007/s13304-015-0297-0. Epub 2015 Apr 21.
10
Upregulation of lncRNA MEG3 Promotes Osteogenic Differentiation of Mesenchymal Stem Cells From Multiple Myeloma Patients By Targeting BMP4 Transcription.上调长链非编码 RNA MEG3 通过靶向 BMP4 转录促进多发性骨髓瘤患者间充质干细胞的成骨分化。
Stem Cells. 2015 Jun;33(6):1985-97. doi: 10.1002/stem.1989.
Zotero 插件