Bareford D, Jennings P E, Stone P C, Baar S, Barnett A H, Stuart J
J Clin Pathol. 1986 Jul;39(7):722-7. doi: 10.1136/jcp.39.7.722.
Erythrocyte deformability was studied in a total of 83 poorly controlled diabetics (mean blood glucose 12.2 mmol/l) who were divided into three groups, each with matched healthy controls. There was no appreciable difference between diabetics and matched controls regarding the filtration of erythrocytes through 3 micron diameter straight channel pores (25 diabetics) or tortuous channel pores (28 diabetics), or for the measurement of erythrocyte elongation over a range of osmolalities in the Ektacytometer (30 diabetics). When erythrocytes from 17 additional diabetics and 17 healthy controls were incubated for two hours at 37 degrees C in hyperglycaemic (50 mmol glucose/l) buffer, however, there was a considerable reduction in erythrocyte filterability for both diabetics and controls in parallel with an increase in erythrocyte sorbitol concentration. This loss of filterability was prevented by the addition of an aldose reductase inhibitor (Sorbinil). High glucose concentrations (congruent to 50 mmol/l) impair the filterability of erythrocytes through 3 micron pores, and the intracellular accumulation of sorbitol in poorly controlled outpatients is therefore unlikely to have a major adverse effect on erythrocyte rheology in diabetes mellitus.
对总共83名血糖控制不佳的糖尿病患者（平均血糖12.2 mmol/l）的红细胞变形性进行了研究，这些患者被分为三组，每组都有匹配的健康对照。在通过3微米直径的直通道孔（25名糖尿病患者）或曲折通道孔（28名糖尿病患者）对红细胞进行过滤方面，以及在Ektacytometer中测量一系列渗透压下红细胞伸长率时（30名糖尿病患者），糖尿病患者与匹配的对照组之间没有明显差异。然而，当另外17名糖尿病患者和17名健康对照的红细胞在高血糖（50 mmol葡萄糖/l）缓冲液中于37摄氏度孵育两小时后，糖尿病患者和对照组的红细胞过滤性均显著降低，同时红细胞山梨醇浓度增加。添加醛糖还原酶抑制剂（索比尼尔）可防止这种过滤性的丧失。高葡萄糖浓度（相当于50 mmol/l）会损害红细胞通过3微米孔的过滤性，因此，在血糖控制不佳的门诊患者中，山梨醇在细胞内的积累不太可能对糖尿病患者的红细胞流变学产生重大不利影响。
