Rillaerts E G, Vertommen J J, De Leeuw I H
Laboratory for Endocrinology and Nutrition, University of Antwerp, Belgium.
Diabetes. 1988 Apr;37(4):471-5. doi: 10.2337/diab.37.4.471.
The hypothesis that sorbitol accumulation could contribute to a reduced erythrocyte deformability in diabetes was investigated. Erythrocyte sorbitol and erythrocyte viscosity at high and low shear rates were studied in 20 insulin-dependent diabetic (IDDM) and 20 matched control subjects. An increased erythrocyte sorbitol and an increased low-shear erythrocyte viscosity were found in the IDDM patients, but there was no significant correlation (r = .11, NS) between the parameters. Incubation (3 h, 37 degrees C) in a Krebs buffer containing 33.3 mM glucose resulted in a significant sorbitol accumulation, but erythrocyte viscosity was not affected. Despite this fact, addition of 1 mM statil (ICI 128436) in the 5.5- and 33.3-mM glucose media not only prevented erythrocyte sorbitol accumulation but also improved erythrocyte viscosity in diabetic and control subjects. The effect was more pronounced at the low (approximately 16%) than at the high (approximately 2%) shear rate. The effect on erythrocyte viscosity disappeared by washing the erythrocytes after incubation, although erythrocyte sorbitol remained different. Our results suggest that sorbitol accumulation does not contribute to an increased erythrocyte viscosity in diabetes, and statil shows a positive effect on erythrocyte viscosity independent of its aldose reductase-inhibiting property.
研究了山梨醇蓄积可能导致糖尿病患者红细胞变形性降低这一假说。对20名胰岛素依赖型糖尿病（IDDM）患者和20名配对的对照受试者进行了高、低剪切率下红细胞山梨醇和红细胞黏度的研究。在IDDM患者中发现红细胞山梨醇增加和低剪切率下红细胞黏度增加，但这些参数之间无显著相关性（r = 0.11，无统计学意义）。在含33.3 mM葡萄糖的Krebs缓冲液中孵育（3小时，37℃）导致显著的山梨醇蓄积，但红细胞黏度未受影响。尽管如此，在5.5 mM和33.3 mM葡萄糖培养基中添加1 mM司他立（ICI 128436）不仅可防止糖尿病患者和对照受试者的红细胞山梨醇蓄积，还可改善红细胞黏度。在低剪切率（约16%）时的作用比高剪切率（约2%）时更明显。孵育后洗涤红细胞，对红细胞黏度的作用消失，尽管红细胞山梨醇仍有差异。我们的结果表明，山梨醇蓄积并非导致糖尿病患者红细胞黏度增加的原因，司他立对红细胞黏度有积极作用，且与其醛糖还原酶抑制特性无关。
