Čermáková Petra, Kovalinka Tomáš, Ferenczyová Kristína, Horváth Anton
Department of Biochemistry, Faculty of Natural Sciences, Comenius University, Bratislava, Slovakia.
Parasite. 2019;26:17. doi: 10.1051/parasite/2019017. Epub 2019 Mar 22.
The measurement of respiratory chain enzyme activities is an integral part of basic research as well as for specialized examinations in clinical biochemistry. Most of the enzymes use ubiquinone as one of their substrates. For current in vitro measurements, several hydrophilic analogues of native ubiquinone are used depending on the enzyme and the workplace. We tested five readily available commercial analogues and we showed that Coenzyme Q is the most suitable for the measurement of all tested enzyme activities. Use of a single substrate in all laboratories for several respiratory chain enzymes will improve our ability to compare data, in addition to simplifying the stock of chemicals required for this type of research.
呼吸链酶活性的测定是基础研究以及临床生物化学专业检查的一个组成部分。大多数酶将泛醌用作其底物之一。对于当前的体外测量，根据所检测的酶和工作场所，使用了几种天然泛醌的亲水性类似物。我们测试了五种容易获得的商业类似物，结果表明辅酶Q最适合用于测定所有测试的酶活性。在所有实验室中对几种呼吸链酶使用单一底物，除了简化这类研究所需的化学试剂储备外，还将提高我们比较数据的能力。
