CEB - Centre of Biological Engineering, University of Minho, 4710-057, Braga, Portugal.
Department of Animal Science, CITAB - Centre for the Research and Technology of Agro-Environmental and Biological Sciences, University of Trás-os-Montes and Alto Douro, 5001-801, Vila Real, Portugal.
Mycotoxin Res. 2019 Aug;35(3):243-252. doi: 10.1007/s12550-019-00349-9. Epub 2019 Mar 21.
This work characterizes the adsorption of aflatoxin B (AFB), ochratoxin A (OTA), and zearalenone (ZEN) by dry micronized olive pomace (OliPom) and grape stems (GrapStem). Their performance was compared with that of three other materials, activated carbon (ActCarb), bentonite (Bent), and a commercial product (ComProd). Experiments were conducted in vitro at several pH values using buffer solutions. For OTA and ZEA, the strongest adsorbent was ActCarb, with 5 mg/mL being sufficient to bind > 99% of all the mycotoxins. For AFB, ComProd and Bent were the most effective adsorbents, as 0.5 mg/mL bound > 95% of this mycotoxin. Among the two agro by-products, GrapStem was the strongest binder, with 10 mg/mL being sufficient to bind at least 90% of all the mycotoxins (except OTA at pH 7). OliPom was the least efficient material, but at a concentration of 30 mg/mL, its performance was similar to GrapStem. Adsorption isotherms were calculated, and ActCarb showed the maximum adsorption capacity (Q), with values that ranged from 19 to 24 μg/mg for pH 2 and from 17 to 20 μg/mg for pH 7. ComProd, Bent, and GrapStem showed more similar Q between them (1.4-4.4 μg/mg for pH 2 and 0.5-4.8 μg/mg for pH 7).
本研究对干燥的微米化橄榄渣（OliPom）和葡萄秸秆（GrapStem）对黄曲霉毒素 B（AFB）、赭曲霉毒素 A（OTA）和玉米赤霉烯酮（ZEN）的吸附特性进行了描述。将其性能与另外三种材料（活性炭（ActCarb）、膨润土（Bent）和商业产品（ComProd））进行了比较。在不同 pH 值下使用缓冲溶液进行了体外实验。对于 OTA 和 ZEA，最强的吸附剂是 ActCarb，5mg/mL 即可结合 >99%的所有霉菌毒素。对于 AFB，ComProd 和 Bent 是最有效的吸附剂，0.5mg/mL 可结合 >95%的这种霉菌毒素。在这两种农业副产品中，GrapStem 是最强的结合剂，10mg/mL 即可结合至少 90%的所有霉菌毒素（pH 7 时除外 OTA）。OliPom 是效率最低的材料，但在 30mg/mL 浓度下，其性能与 GrapStem 相似。计算了吸附等温线，ActCarb 表现出最大的吸附容量（Q），在 pH 2 时范围为 19-24μg/mg，在 pH 7 时范围为 17-20μg/mg。ComProd、Bent 和 GrapStem 之间的 Q 值更为相似（pH 2 时为 1.4-4.4μg/mg，pH 7 时为 0.5-4.8μg/mg）。
