Vazquez-Ortiz Tania Karina, Lozano-Contreras Lisseth, Salazar Ana María, Sordo Monserrat, Figueroa-Cárdenas Juan de Dios, Vázquez-Durán Alma, Méndez-Albores Abraham
Unidad de Investigación Multidisciplinaria L14 (Alimentos, Micotoxinas, y Micotoxicosis), Facultad de Estudios Superiores Cuautitlán (FESC), Universidad Nacional Autónoma de México (UNAM), 54714, Cuautitlán Izcalli, Estado de México, Mexico.
Instituto de Ciencias Agropecuarias, Universidad Autónoma del Estado de Hidalgo, Av. Universidad Km. 1, 43600, Hidalgo, Mexico.
Mycotoxin Res. 2025 May;41(2):373-383. doi: 10.1007/s12550-025-00588-z. Epub 2025 Mar 20.
A viable strategy for addressing the aflatoxin issue using two enterosorbents prepared from marigold petals and guava leaves was validated. The enterosorbents were characterized via Fourier transform infrared spectroscopy (FTIR), field-emission scanning electron microscopy (FESEM), energy dispersive X-ray fluorescence spectroscopy (EDS), and X-ray diffraction (XRD) to obtain information about the surface functional groups, microstructure, multi-elemental composition, degree of crystallinity, and phase analysis. The potential of the enterosorbents in decreasing aflatoxin uptake and bioavailability under simulated gastrointestinal conditions (including the replication of chemical and enzymatic factors) was estimated using the isotherm models of Langmuir, Freundlich, Temkin, and Dubinin-Radushkevich. Under the simulated gastric and intestinal conditions, marigold removed almost all the mycotoxin at doses of 0.25 and 0.125% (w/w); however, guava leaves efficiently adsorbed the toxin when using doses up to 0.5 and 0.25% (w/w), respectively. Equilibrium adsorption data followed preferentially the Freundlich model, the values of the Freundlich constant (K) for marigold were 37.3 and 7.1 times higher than those of guava leaves, respectively. Additionally, the n value was > 1, indicative that adsorption was mainly dominated by physical mechanisms. Overall, this research provides insights into the practical application of natural enterosorbents offering a promising approach for AFB removal.
一种使用从万寿菊花瓣和番石榴叶制备的两种肠吸附剂来解决黄曲霉毒素问题的可行策略得到了验证。通过傅里叶变换红外光谱（FTIR）、场发射扫描电子显微镜（FESEM）、能量色散X射线荧光光谱（EDS）和X射线衍射（XRD）对肠吸附剂进行了表征，以获取有关表面官能团、微观结构、多元素组成、结晶度和相分析的信息。使用朗缪尔、弗伦德利希、坦金和杜比宁-拉杜舍维奇等温线模型估计了肠吸附剂在模拟胃肠道条件下（包括化学和酶促因素的复制）降低黄曲霉毒素摄取和生物利用度的潜力。在模拟的胃和肠道条件下，万寿菊在0.25%和0.125%（w/w）的剂量下几乎去除了所有霉菌毒素；然而，番石榴叶分别在高达0.5%和0.25%（w/w）的剂量下有效地吸附了毒素。平衡吸附数据优先遵循弗伦德利希模型，万寿菊的弗伦德利希常数（K）值分别比番石榴叶高37.3倍和7.1倍。此外，n值>1，表明吸附主要由物理机制主导。总体而言，本研究为天然肠吸附剂的实际应用提供了见解，为去除黄曲霉毒素提供了一种有前景的方法。
