Daspute Abhijit A, Yunxuan Xian, Gu Minghua, Kobayashi Yuriko, Wagh Sopan, Panche Archana, Koyama Hiroyuki
Laboratory of Plant Cell Technology, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan.
Institute of Bioscience & Biotechnology, Department of Biological Sciences, MGM College, Aurangabad 411-003, India.
Future Sci OA. 2019 Feb 8;5(3):FSO364. doi: 10.4155/fsoa-2018-0065. eCollection 2019 Mar.
To develop a useful alternative approach to evaluate the gene function in hairy roots.
and tobacco (wild-type or mutant) were a host for transformation.
The hairy roots formation efficiency ranged from 53 to 98% in tobacco and 53 to 66% in . Hairy and intact roots showed similar gene expression pattern in response to salt and aluminum stress. Genomic polymerase chain reaction and fluorescent images showed high rate (>80%) of co-integration of T-DNAs and uniform cell transformation without use of any antibiotic selection. Whole processes of hairy roots were completed within 1 month after the infection of .
Aluminum-responsive orthologous gene function could be evaluated by KD and as a host for hairy roots transformation.
开发一种有用的替代方法来评估毛状根中的基因功能。
以（未提及具体植物，可能有遗漏）和烟草（野生型或突变体）作为转化宿主。
烟草中毛状根形成效率在53%至98%之间，（此处缺失一种植物名称）中在53%至66%之间。毛状根和完整根在响应盐和铝胁迫时表现出相似的基因表达模式。基因组聚合酶链反应和荧光图像显示T-DNA共整合率高（>80%），且无需使用任何抗生素选择即可实现均匀的细胞转化。在（未提及具体感染对象，可能有遗漏）感染后1个月内完成了毛状根的整个过程。
铝响应直系同源基因功能可通过KD（未提及具体含义，可能有遗漏）评估，且（未提及具体植物，可能有遗漏）可作为毛状根转化的宿主。
