介导的毛状根转化作为探索铝响应基因功能的工具。
-mediated hairy roots transformation as a tool for exploring aluminum-responsive genes function.
作者信息
Daspute Abhijit A, Yunxuan Xian, Gu Minghua, Kobayashi Yuriko, Wagh Sopan, Panche Archana, Koyama Hiroyuki
机构信息
Laboratory of Plant Cell Technology, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan.
Institute of Bioscience & Biotechnology, Department of Biological Sciences, MGM College, Aurangabad 411-003, India.
出版信息
Future Sci OA. 2019 Feb 8;5(3):FSO364. doi: 10.4155/fsoa-2018-0065. eCollection 2019 Mar.
AIM
To develop a useful alternative approach to evaluate the gene function in hairy roots.
METHODS
and tobacco (wild-type or mutant) were a host for transformation.
RESULTS
The hairy roots formation efficiency ranged from 53 to 98% in tobacco and 53 to 66% in . Hairy and intact roots showed similar gene expression pattern in response to salt and aluminum stress. Genomic polymerase chain reaction and fluorescent images showed high rate (>80%) of co-integration of T-DNAs and uniform cell transformation without use of any antibiotic selection. Whole processes of hairy roots were completed within 1 month after the infection of .
CONCLUSION
Aluminum-responsive orthologous gene function could be evaluated by KD and as a host for hairy roots transformation.
目的
开发一种有用的替代方法来评估毛状根中的基因功能。
方法
以（未提及具体植物，可能有遗漏）和烟草（野生型或突变体）作为转化宿主。
结果
烟草中毛状根形成效率在53%至98%之间，（此处缺失一种植物名称）中在53%至66%之间。毛状根和完整根在响应盐和铝胁迫时表现出相似的基因表达模式。基因组聚合酶链反应和荧光图像显示T-DNA共整合率高（>80%），且无需使用任何抗生素选择即可实现均匀的细胞转化。在（未提及具体感染对象，可能有遗漏）感染后1个月内完成了毛状根的整个过程。
结论
铝响应直系同源基因功能可通过KD（未提及具体含义，可能有遗漏）评估，且（未提及具体植物，可能有遗漏）可作为毛状根转化的宿主。
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