Chemical Engineering Department, Federal University of Rio Grande do Sul, Engenheiro Luiz Englert Street, Porto Alegre, RS 90040-040, Brazil.
Institute of Food Science and Technology, Federal University of Rio Grande do Sul, Bento Gonçalves Avenue, 9500, Porto Alegre, RS 91501-970, Brazil.
Bioresour Technol. 2019 Jul;283:207-212. doi: 10.1016/j.biortech.2019.03.035. Epub 2019 Mar 8.
Pulsed electric field (PEF) treatment was evaluated for phycocyanin and proteins extraction from Arthrospira platensis (Spirulina platensis). PEF extractions were performed using different specific energies (28, 56 and 122 J·ml of suspension) and the results were compared to the extraction with bead milling. At highest PEF-treatment energies a damage of the cell morphology could be observed and the highest yields (up to 85.2 ± 5.7 mg·g and 48.4 ± 4.4 g·100 g of phycocyanins and proteins, respectively) could be obtained at 122 and 56 J·ml. The yields increased with incubation time after PEF-treatment. The antioxidant capacity of the extracts obtained after PEF-treatment was higher than of those obtained after bead milling. PEF treatment is a promising technology to obtain blue-green antioxidant extracts from A. platensis in an environmental friendly process.
脉冲电场（PEF）处理用于从螺旋藻（Arthrospira platensis）中提取藻蓝蛋白和蛋白质。使用不同的比能量（28、56 和 122 J·ml 悬浮液）进行了 PEF 提取，并将结果与珠磨提取进行了比较。在最高的 PEF 处理能量下，可以观察到细胞形态的破坏，并且可以在 122 和 56 J·ml 时获得最高的产率（分别高达 85.2±5.7 mg·g 和 48.4±4.4 g·100 g 的藻蓝蛋白和蛋白质）。产率随着 PEF 处理后的孵育时间增加而增加。PEF 处理后获得的提取物的抗氧化能力高于珠磨处理后获得的提取物。PEF 处理是一种很有前途的技术，可以在环保的过程中从 A. platensis 中获得蓝绿色抗氧化提取物。
