The First Affiliated Hospital of Zhengzhou University, No. 1, Jianshe East Road, Zhengzhou 450052, PR China.
Henan Provincial Key Laboratory of Surface and Interface Science, Zhengzhou University of Light Industry, No. 136, Science Avenue, Zhengzhou 450001, PR China.
Biosens Bioelectron. 2019 May 15;133:55-63. doi: 10.1016/j.bios.2019.03.030. Epub 2019 Mar 18.
A bimetallic NiCo-based metal-organic framework (NiCo-MOF) was pyrolyzed into a novel composite comprising NiCoO spinel, CoO, and metallic Co/Ni nanoparticles embedded with carbon nanotubes at high temperature 700 °C under N atmosphere (represented by NiCoO/CoO@CNTs), whereas organic ligands were almost decomposed in H atmosphere, leading to absence of carbon nanotubes (denoted by NiCoO/CoO). The feasibility of using the composite as efficient bioplatform for immobilizing the probe DNA of human immune deficiency virus-1 (HIV-1) in the electrochemical detection of the HIV-1 DNA was explored. Compared with the pristine NiCo-MOF and NiCoO/CoO, the NiCoO/CoO@CNTs composite exhibited high electrochemical activity, good biocompatibility, and strong bioaffinity toward the probe DNA. Electrochemical measurements demonstrated that the NiCoO/CoO@CNTs-based bioassay displayed superior sensing performances, giving an ultralow detection limit of 16.7 fM toward HIV-1 DNA over the linear range of 0.1 pM to 20 nM; possessing high selectivity even against noncomplementary and two-base mismatch sequences; exhibiting good stability, reproducibility, repeatability and applicability in the application of detecting human serum samples. Thus, the present strategy can broaden the application of MOFs and their derivatives in various fields of biosensing.
一种双金属 NiCo 基金属有机骨架（NiCo-MOF）在 N 气氛下于 700°C 高温下热解为一种新型复合材料，包含尖晶石 NiCoO、CoO 和嵌入碳纳米管的金属 Co/Ni 纳米粒子（表示为 NiCoO/CoO@CNTs），而有机配体在 H 气氛中几乎完全分解，导致没有碳纳米管（表示为 NiCoO/CoO）。探索了将该复合材料用作固定人类免疫缺陷病毒-1（HIV-1）探针 DNA 的有效生物平台，用于 HIV-1 DNA 的电化学检测。与原始的 NiCo-MOF 和 NiCoO/CoO 相比，NiCoO/CoO@CNTs 复合材料表现出高电化学活性、良好的生物相容性和对探针 DNA 的强生物亲和力。电化学测量表明，基于 NiCoO/CoO@CNTs 的生物测定法表现出优异的传感性能，对 HIV-1 DNA 的检测限低至 16.7 fM，线性范围为 0.1 pM 至 20 nM；具有高选择性，即使针对非互补和两个碱基错配序列也具有高选择性；表现出良好的稳定性、重现性、可重复性和在检测人血清样本中的适用性。因此，本策略可以拓宽 MOFs 及其衍生物在生物传感等各个领域的应用。
