a The Beijing Key Laboratory of Ophthalmology and Visual Science, Beijing Tongren Eye Center, Beijing Tongren Hospital of Capital Medical University , Beijing , People's Republic of China.
b The Refractive Surgery Center, Yunnan Eye Institute, The Second People's Hospital of Yunnan Province , Kunming , People's Republic of China.
Curr Eye Res. 2019 Aug;44(8):832-839. doi: 10.1080/02713683.2019.1597890. Epub 2019 Apr 18.
: To (i) evaluate various methods for preserving refractive lenticules (RLs) from myopic eyes following small-incision lenticule extraction (SMILE), in order to (ii) establish a sound, standard storage RL preservative for clinical uses. : In this prospective study, we compared freshly excised post-SMILE RLs (control group) with post-SMILE RLs (experimental group). Experimental group RLs were preserved in one of several preservatives: glycerol, allochroic silicagel desiccant, or Optisol. Following preservation in one of these three media, samples were evaluated by light microscopy (LM), and transmission (TEM) and scanning (SEM) electron microscopy on days-1, -3, -7, and -14. : Changes in cellular morphology were observed at all time points. Compared with fresh control-group RLs, there were significant histological changes in RLs preserved in glycerol and allochroic silicagel, but not . Comparison of the three methods revealed to be the best, followed by allochroic silica gel desiccant, followed by glycerol. RLs preserved in maintained the highest degree of viability and integrity. And the RLs viability and collagen density decreased with prolongation of storage time all. : is a midterm corneal storage medium, which can maintain post-SMILE corneal RLs for 14 days, is a feasible and effective method for tissue storage.
（i）评估小切口角膜基质透镜切除术（SMILE）后保存近视眼角膜折射性透镜（RL）的各种方法，以便（ii）为临床应用建立一种可靠、标准的 RL 保存液。
前瞻性研究中，我们比较了新鲜的 SMILE 后 RL（对照组）和 SMILE 后 RL（实验组）。实验组 RL 保存在三种保存液之一：甘油、变色硅胶干燥剂或 Optisol。在这三种介质中保存 1 天后，通过光镜（LM）和透射电镜（TEM）、扫描电镜（SEM）评估样本，第 3、7、14 天再次评估。
所有时间点均观察到细胞形态变化。与新鲜对照组 RL 相比，保存在甘油和变色硅胶中的 RL 有明显的组织学变化，但 Optisol 组无变化。三种方法比较显示 Optisol 最好，其次是变色硅胶干燥剂，最后是甘油。保存在 Optisol 中的 RL 保持了最高的活力和完整性。RL 的活力和胶原密度随保存时间的延长而降低。
Optisol 是一种中期角膜储存介质，可保存 SMILE 后角膜 RL 长达 14 天，是组织储存的一种可行且有效的方法。
