Department of Ophthalmology, University Hospital Kralovske Vinohrady and 3rd Medical Faculty, Srobarova 1150/50, Prague 10, 100 34, Prague, Czech Republic.
Cell Tissue Bank. 2022 Dec;23(4):627-639. doi: 10.1007/s10561-021-09990-0. Epub 2022 Feb 6.
Corneal stromal lenticule is a part of corneal stroma, which can be created by manual dissection, by femtosecond laser from the donor cornea, but chiefly it is a waste product of a refractive procedure ReLex SMILE (Small Incision Lenticule Extraction). Corneal lenticule has a huge potential in corneal surgery. In recent years, many studies have been published to show the possibility to use this tissue to treat corneal defects, as well as in refractive surgery. Thanks to the quantity of lenticules which arise every day during SMILE operations, this tissue is much more accessible than any other kind of corneal tissue. According to the experience with lenticule implantation in animal models, or even human patients, lenticule implantation is considered safe, reversible method, which is not associated with immune rejection or other severe complications. However, the crucial step before the process of lenticule implantation, is proper preservation of this tissue. Donor corneal tissue containing endothelium is usually preserved in hypothermia and then usable maximally for two weeks. Newer methods such as organ culture storage and use of a sterile cornea prolong the time of usability of the tissue. The possibilities for corneal lenticule storage are theoretically wider thanks to the fact, that we do not need to preserve fragile cellular structures. Besides the storage in hypothermia, other preserving methods such as cryopreservation and storage after decellularization have been tested. This review aimed to examine the current literature that describes possible methods of corneal lenticule preservation. A comprehensive search was created based on articles published in English on PubMed.gov, Cochranelibrary.com and Scopus.com using following keywords: corneal lenticule preservation, corneal lenticule storage, cold storage corneal lenticule, corneal lenticule cryopreservation till 2020.
角膜基质透镜是角膜基质的一部分，可以通过手动解剖、飞秒激光从供体角膜获得，但主要是屈光手术 ReLex SMILE（小切口透镜提取）的废物。角膜透镜在角膜手术中有巨大的潜力。近年来，许多研究已经发表，表明使用这种组织治疗角膜缺陷以及屈光手术的可能性。由于 SMILE 手术每天都会产生大量的透镜，因此这种组织比任何其他类型的角膜组织都更容易获得。根据动物模型甚至人类患者的透镜植入经验，透镜植入被认为是一种安全、可逆转的方法，不会引起免疫排斥或其他严重并发症。然而，在进行透镜植入之前，关键步骤是正确保存这种组织。含有内皮的供体角膜组织通常在低温下保存，然后最多可使用两周。器官培养储存和使用无菌角膜等较新的方法可延长组织的使用时间。由于我们不需要保存脆弱的细胞结构，因此角膜透镜的储存可能性在理论上更广泛。除了低温保存外，还测试了其他保存方法，如冷冻保存和去细胞化后保存。本综述旨在检查描述角膜透镜保存的可能方法的现有文献。根据在 PubMed.gov、CochraneLibrary.com 和 Scopus.com 上发表的英文文章，使用以下关键字创建了全面的搜索：角膜透镜保存、角膜透镜储存、冷藏角膜透镜、角膜透镜冷冻保存至 2020 年。
