Eye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan University, Shanghai, China; NHC Key Laboratory of Myopia (Fudan University), Key Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, China; Shanghai Research Center of Ophthalmology and Optometry, Shanghai, China.
Eye Institute and Department of Ophthalmology, Eye & ENT Hospital, Fudan University, Shanghai, China; NHC Key Laboratory of Myopia (Fudan University), Key Laboratory of Myopia, Chinese Academy of Medical Sciences, Shanghai, China; Shanghai Research Center of Ophthalmology and Optometry, Shanghai, China.
Exp Eye Res. 2020 Dec;201:108357. doi: 10.1016/j.exer.2020.108357. Epub 2020 Nov 13.
To investigate the optical transmittance and ultrastructure of small incision lenticule extraction (SMILE)-derived lenticules preserved via three different methods.
A total of 90 lenticules obtained from myopic patients undergoing SMILE surgery were divided into control and experimental groups. Fresh lenticules served as the control. The preserved lenticules of the experimental group were randomly divided into three subgroups according to different storage conditions: anhydrous glycerol, silicone oil and allochroic silica gel groups. Optical transmittance was evaluated, histological changes were analysed by haematoxylin eosin (HE) staining, and collagen fibril densities and necrotic response were assessed via transmission electron microscopy (TEM) at 48 h, 14 days and 4 weeks.
After storage for 4 weeks, the mean percentage transmittance values in glycerol and silicone oil groups significantly decreased (P = 0.034 and P = 0.042, respectively), but the lenticules preserved in silica gel remained unchanged when compared with the control lenticules. In all the groups, HE staining results showed a regular arrangement of collagen fibers with a few keratocytes and several cavitation bubbles. TEM revealed that the fibril densities in the glycerol group (273.70 ± 31.42/μm) after 4 weeks were significant less than those in the other two groups (silicone oil, 335.20 ± 33.09/μm; silica gel, 371.80 ± 37.60/μm) and control group (340 ± 33.61/μm) (all P < 0.001). In each group, a few necrotic and apoptotic keratocytes were observed.
All the three agents, namely glycerol, silicone oil and silica gel, could be used for lenticule preservation. Silica gel facilitates better maintenance of optical transmittance than the other two agents.
研究通过三种不同方法保存的小切口透镜提取（SMILE）衍生透镜的透光率和超微结构。
共收集 90 个来自接受 SMILE 手术的近视患者的透镜，分为对照组和实验组。新鲜透镜作为对照组。实验组的保存透镜根据不同的储存条件随机分为三组：无水甘油组、硅油组和变色硅胶组。在 48 小时、14 天和 4 周时评估透光率，通过苏木精-伊红（HE）染色分析组织学变化，并通过透射电子显微镜（TEM）评估胶原纤维密度和坏死反应。
保存 4 周后，甘油组和硅油组的平均透光率百分比显著降低（P=0.034 和 P=0.042），而硅胶组保存的透镜与对照组相比没有变化。在所有组中，HE 染色结果显示胶原纤维排列规则，有少量角膜细胞和几个空泡。TEM 显示，甘油组（4 周后 273.70±31.42/μm）的纤维密度明显低于其他两组（硅油组 335.20±33.09/μm；硅胶组 371.80±37.60/μm）和对照组（340±33.61/μm）（均 P<0.001）。在每组中，观察到少量坏死和凋亡的角膜细胞。
甘油、硅油和硅胶这三种试剂均可用于透镜保存。硅胶比其他两种试剂更有利于保持透光率。
