Mendonça Soares Suelen, Domingues Robert, Baldo Gaspar Emanuelle, Azevedo Dos Santos Patrício, Marques Canuto Kirley, Pelegrine Minho Alessandro, Botelho Vieira Maria Isabel
Programa de Pós-Graduação em Bioexperimentação, Universidade de Passo Fundo, Passo Fundo, Rio Grande do Sul, Brasil.
Embrapa Pecuária Sul, Bagé, Rio Grande do Sul, Brasil.
BMC Vet Res. 2019 Mar 25;15(1):99. doi: 10.1186/s12917-019-1843-7.
Haemonchosis affects sheep husbandry and its treatment is often compromised due to the development of anthelminthic resistance. Plant-derived bioactive compounds have been studied as alternative to control Haemonchus contortus. The objective of this study was to evaluate the effect of Senecio brasiliensis extracts on H. contortus egg hatching and infective larvae migration.
The aqueous extract from dried and fresh plant and alkaloid-enriched fraction of the previously dried leaves of S. brasiliensis inhibited H. contortus egg hatching. The main plant compound in alkaloid fraction was integerrimine, a pyrrolizidine alkaloid (PA). However, the aqueous extract from dried plant displayed higher efficacy when compared to their alkaloid enriched or non-polar fractions, meaning that, although PAs contributed to the ovicidal effect, other compounds in the plant can also contribute to their effect. Furthermore, the aqueous extract from dried plant also had higher efficacy than aqueous extract from fresh plant in larvae migration inhibition. Finally, extract from dried plant presented low in vitro cytotoxic effect.
Taken together our results suggest a good anthelmintic effect of S. brasiliensis, especially when aqueous extract is prepared from dried plant. Further in vivo studies should be performed focused on forms of administration of this extract in rearing sheep.
捻转血矛线虫病影响养羊业，由于抗蠕虫药耐药性的发展，其治疗常常受到影响。植物源生物活性化合物已作为控制捻转血矛线虫的替代物进行了研究。本研究的目的是评估巴西千里光提取物对捻转血矛线虫虫卵孵化和感染性幼虫迁移的影响。
巴西千里光干燥和新鲜植物的水提取物以及先前干燥叶片的富含生物碱部分抑制了捻转血矛线虫虫卵的孵化。生物碱部分的主要植物化合物是全缘千里光碱，一种吡咯里西啶生物碱（PA）。然而，与富含生物碱或非极性部分相比，干燥植物的水提取物显示出更高的功效，这意味着，虽然PA有助于杀卵作用，但植物中的其他化合物也可能有助于其作用。此外，干燥植物的水提取物在抑制幼虫迁移方面也比新鲜植物的水提取物具有更高的功效。最后，干燥植物提取物在体外显示出低细胞毒性作用。
综合我们的结果表明巴西千里光具有良好的驱虫效果，特别是当从干燥植物中制备水提取物时。应进一步开展体内研究，重点关注该提取物在饲养绵羊中的给药形式。
