Laboratory of Cardiovascular Disease, Taizhou Hospital, Wenzhou Medical University, Taizhou, China.
Enze Medical Research Center, Taizhou, China.
J Clin Lab Anal. 2019 Jun;33(5):e22881. doi: 10.1002/jcla.22881. Epub 2019 Mar 26.
To assess the association of gene polymorphisms of angiotensinogen (AGT), the key factor in rennin-angiotensin-aldosterone system (RAAS), with high-sensitivity C-reactive protein (hs-CRP) and coronary artery disease (CAD).
The current study recruited the patients who were hospitalized and assessed by coronary angiography for suspected CAD. The patients with documented CAD served as CAD group (n = 492) while the patients without documented CAD (n = 87) served as control group. We compared laboratory data and CAD risk factors between the two groups. Furthermore, we analyzed the association of AGT M235T, G217A, G152A, G-6A, A-20C genotypes with coronary artery stenosis and in-stent restenosis.
There were significantly differences between two patient groups in sex, smoking history, diabetes mellitus, carotid atherosclerosis, lower limb arteriosclerosis, hs-CRP, blood glucose, and the level of high-density lipoprotein (HDL; P < 0.05). In CAD group, hs-CRP levels increased with increasing number of coronary artery branches (1, 2, or ≥3; P < 0.01), and Gensini integral was positively correlated with hs-CRP levels (r = 0.361, P < 0.01). Frequencies of genotype and allele distribution in individual angiotensinogen loci (M235T, G217A, G152A, G-6A, A-20C) did not differ in two patient groups. Following stratification of patients according to hs-CRP levels (<1 mg/L, 1-3 mg/L, and >3 mg/L), the distribution frequency of allele M235T was statistically different among the groups (P < 0.05).
In CAD patients, M235T among several AGT gene polymorphisms is associated with elevated hs-CRP levels with AGT C allele as the significant factor for patients with hs-CRP level of more than 1 mg/L.
评估血管紧张素原（AGT）基因多态性（肾素-血管紧张素-醛固酮系统（RAAS）的关键因素）与高敏 C 反应蛋白（hs-CRP）和冠心病（CAD）之间的关联。
本研究招募了因疑似 CAD 而行冠状动脉造影评估的住院患者。有 CAD 病史的患者作为 CAD 组（n=492），无 CAD 病史的患者（n=87）作为对照组。我们比较了两组之间的实验室数据和 CAD 危险因素。此外，我们分析了 AGT M235T、G217A、G152A、G-6A、A-20C 基因型与冠状动脉狭窄和支架内再狭窄的关系。
两组患者在性别、吸烟史、糖尿病、颈动脉粥样硬化、下肢动脉硬化、hs-CRP、血糖和高密度脂蛋白（HDL）水平等方面存在显著差异（P<0.05）。在 CAD 组中，随着冠状动脉分支数量的增加（1、2 或≥3），hs-CRP 水平逐渐升高（P<0.01），且 Gensini 积分与 hs-CRP 水平呈正相关（r=0.361，P<0.01）。两组患者在单个血管紧张素原基因座（M235T、G217A、G152A、G-6A、A-20C）的基因型和等位基因分布频率无差异。根据 hs-CRP 水平（<1mg/L、1-3mg/L 和>3mg/L）对患者进行分层后，各组之间等位基因 M235T 的分布频率存在统计学差异（P<0.05）。
在 CAD 患者中，AGT 基因多态性中的 M235T 与升高的 hs-CRP 水平相关，AGT C 等位基因是 hs-CRP 水平>1mg/L 患者的重要因素。
