Devasagayam T P
FEBS Lett. 1986 Sep 15;205(2):246-50. doi: 10.1016/0014-5793(86)80906-1.
Senescence is associated with decrease in the NADPH-induced lipid peroxidation in liver homogenate as well as rough and smooth microsomes of female rats. In the microsomal fractions, sensitivity to NADPH-induced lipid peroxidation is high in young adults (3-month-old), decreases in middle aged (12-month-old) and reaches lowest levels in senescent (30-month-old) rats. Increasing the concentration of co-factors or time of incubation does not alter this resistance observed in the senescent rats. Major factors responsible for this resistance in senescent rats seem to be low levels of substrate in the c reductase, cytochrome P-450 and high cholesterol:phospholipid ratios besides enhanced levels of superoxide dismutase, alpha-tocopherol and reduced glutathione.
衰老与雌性大鼠肝脏匀浆以及粗面和滑面微粒体中NADPH诱导的脂质过氧化作用降低有关。在微粒体部分，对NADPH诱导的脂质过氧化作用的敏感性在年轻成年大鼠（3个月大）中较高，在中年大鼠（12个月大）中降低，并在衰老大鼠（30个月大）中达到最低水平。增加辅因子浓度或孵育时间不会改变衰老大鼠中观察到的这种抗性。衰老大鼠中这种抗性的主要原因似乎是c还原酶、细胞色素P-450中的底物水平较低，胆固醇与磷脂的比例较高，此外超氧化物歧化酶、α-生育酚和还原型谷胱甘肽的水平也有所提高。
