Devasagayam T P, Pushpendran C K, Eapen J
Biochim Biophys Acta. 1983 Jan 7;750(1):91-7. doi: 10.1016/0005-2760(83)90207-2.
The rough and smooth microsomes of rat liver show significant differences in lipid peroxidation induced by both NADPH and ascorbate. The parameters studied include kinetics, response towards cofactors and sensitivity to inhibitors. Smooth microsomes are more prone to lipid peroxidation with increasing concentrations of NADPH, Fe3+, ascorbate and Fe2+, and are more susceptible to inhibitors than rough microsomes. Smooth microsomes also contain higher amounts of ascorbic acid, NADPH cytochrome c reductase and total lipids, besides possessing a higher degree of unsaturation in lipids, all of which promote lipid peroxidation. Our results suggest that, although smooth microsomes are more sensitive to lipid peroxidation, they are compensated for by being more sensitive to inhibitors of lipid peroxidation.
大鼠肝脏的粗面微粒体和滑面微粒体在由NADPH和抗坏血酸诱导的脂质过氧化方面表现出显著差异。所研究的参数包括动力学、对辅因子的反应以及对抑制剂的敏感性。随着NADPH、Fe3 +、抗坏血酸和Fe2 +浓度的增加，滑面微粒体更容易发生脂质过氧化，并且比粗面微粒体对抑制剂更敏感。除了脂质具有更高程度的不饱和度外，滑面微粒体还含有更高含量的抗坏血酸、NADPH细胞色素c还原酶和总脂质，所有这些都促进脂质过氧化。我们的结果表明，尽管滑面微粒体对脂质过氧化更敏感，但它们对脂质过氧化抑制剂更敏感，从而得到了补偿。
