Sadeghzadeh Firouzeh, Sadeghzadeh Azizeh, Changizi-Ashtiyani Saeed, Bakhshi Sepideh, Mashayekhi Farideh Jalali, Mashayekhi Mehry, Poorcheraghi Hossein, Zarei Ali, Jafari Mostafa
Traditional and Complementary Medicine Research Center, Arak University of Medical Sciences, Arak, Iran.
Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran.
Int J Reprod Biomed. 2020 Apr 30;18(4):295-306. doi: 10.18502/ijrm.v13i4.6892. eCollection 2020 Apr.
The aim of this study was to investigate the antioxidant effects of extract on improving the toxicity induced by cyclophosphamide (CP) on spermatogenesis. 54 male Wistar rats (4 months old) weighing 200-250 gr were randomly divided into 6 groups (n = 9/each).
"group 1 (control) underwent the normal diet and water; group 2 (sham) received 2 ml/day normal saline; group 3 (positive control) received 300 mg/kg/day extract; group 4 received extract (300 mg/kg/day) + 5 mg/kg/day CP (Endoxan, baxter oncology gmbh, Germany) after 4 hr; group 5 (CP) received 5 mg/kg/day CP + normal saline 4 hr after it; and group 6 (CP + ) received extract (300 mg/kg/day) 4 hr after 5 mg/kg/day CP." 24 hr after the last gavage, heart blood sampling was performed to measure the levels of malondialdehyde (MDA), ferric reducing antioxidant power, testosterone, luteinizing hormone, and follicle-stimulating hormone. The left caudal epididymis was cut in the Ham's F10 and the released spermatozoa were used to analyze sperm parameters. The histology of the right testes was studied using stereological techniques and the left testes were used to measure the level of tissue MDA and ferric reducing antioxidant power.
A significant increase in the mean level of MDA (p = 0.013) was seen in the CP compared to the control group. Sperm motility (p = 0.001) and count (p = 0.002), serum and tissue total antioxidant (p 0.001) and serum testosterone levels (p = 0.019) decreased in the CP compared to the control group. extract could significantly prevent the adverse effects of CP on sperm motility (p < 0.001), the mean levels of tissue MDA (p = 0.018), serum total antioxidant (p = 0.045), and testosterone (p < 0.001).
The extract can modify the reproductive toxicity of CP in rat due to the presence of antioxidant compounds.
本研究旨在探讨提取物对改善环磷酰胺（CP）诱导的精子发生毒性的抗氧化作用。将54只体重200 - 250克、4月龄的雄性Wistar大鼠随机分为6组（每组n = 9）。
“第1组（对照组）给予正常饮食和饮水；第2组（假手术组）每天接受2毫升生理盐水；第3组（阳性对照组）每天接受300毫克/千克提取物；第4组在4小时后接受提取物（300毫克/千克/天）+ 5毫克/千克/天CP（德国百特肿瘤公司生产的环磷酰胺）；第5组（CP组）在给予5毫克/千克/天CP 4小时后接受生理盐水；第6组（CP +组）在给予5毫克/千克/天CP 4小时后接受提取物（300毫克/千克/天）。”在最后一次灌胃24小时后，采集心脏血液以测量丙二醛（MDA）、铁还原抗氧化能力、睾酮、黄体生成素和卵泡刺激素的水平。将左侧附睾尾部在哈姆氏F10培养基中切开，释放的精子用于分析精子参数。使用体视学技术研究右侧睾丸的组织学，左侧睾丸用于测量组织MDA水平和铁还原抗氧化能力。
与对照组相比，CP组MDA平均水平显著升高（p = 0.013）。与对照组相比，CP组精子活力（p = 0.001）、精子计数（p = 0.002）、血清和组织总抗氧化能力（p < 0.001）以及血清睾酮水平（p = 0.019）均降低。提取物可显著预防CP对精子活力（p < 0.001）、组织MDA平均水平（p = 0.018）、血清总抗氧化能力（p = 0.045）和睾酮（p < 0.001）的不良影响。
由于存在抗氧化化合物，该提取物可改变CP对大鼠的生殖毒性。
