Peña-Montes Donovan J, Huerta-Cervantes Maribel, Ríos-Silva Mónica, Trujillo Xóchitl, Huerta Miguel, Noriega-Cisneros Ruth, Salgado-Garciglia Rafael, Saavedra-Molina Alfredo
Instituto de Investigaciones Químico Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, 58030 Morelia, Mich., Mexico.
Centro Universitario de Investigaciones Biomédicas, Universidad de Colima, 28040 Colima, Col., Mexico.
Antioxidants (Basel). 2019 Mar 26;8(3):73. doi: 10.3390/antiox8030073.
In the present study, we investigated the composition and antioxidant activity of the hexanic extract of inflorescences by employing in vitro assays to measure antioxidant capacity and 2,2-diphenyl-1-picrylhydrazyl scavenging activity. We also applied the hexanic extract to , under hydrogen peroxide-induced stress. Finally, we tested the extract in male Wistar rats with and without streptozotocin-induced diabetes. The compounds in the hexanic extract were analyzed by gas-chromatography-mass spectrometry, which revealed mainly terpenes and sesquiterpenes, including (Z)β-farnesene (38.79%), β-pinene (17.53%), calamene (13.3%), and α-farnesene (10.38%). In vitro and in , the extract possessed antioxidant activity at different concentrations, compared to ascorbic acid (positive control). In normoglycemic and hyperglycemic rats, oral administration of 30 mg/kg of the extract reduced blood glucose levels; lipid peroxidation in liver, kidney and brain; protein carbonylation; and reactive oxygen species (ROS) production. It also increased catalase activity in the brain, kidneys and liver. These findings show that this hexanic extract of inflorescences possessed antioxidant properties.
在本研究中，我们通过体外试验来测量抗氧化能力和2,2-二苯基-1-苦基肼清除活性，从而研究了花序己烷提取物的成分和抗氧化活性。我们还将己烷提取物应用于过氧化氢诱导的应激条件下。最后，我们在有和没有链脲佐菌素诱导糖尿病的雄性Wistar大鼠中测试了该提取物。通过气相色谱-质谱联用仪分析了己烷提取物中的化合物，结果显示主要为萜类和倍半萜类，包括（Z）β-法尼烯（38.79%）、β-蒎烯（17.53%）、杜松烯（13.3%）和α-法尼烯（10.38%）。在体外试验和体内试验中，与抗坏血酸（阳性对照）相比，该提取物在不同浓度下均具有抗氧化活性。在血糖正常和高血糖的大鼠中，口服30mg/kg的提取物可降低血糖水平；降低肝脏、肾脏和大脑中的脂质过氧化；降低蛋白质羰基化；减少活性氧（ROS）的产生。它还增加了大脑、肾脏和肝脏中的过氧化氢酶活性。这些发现表明这种花序己烷提取物具有抗氧化特性。
