Friedlander A, Rimon A, Lengy J
Z Parasitenkd. 1986;72(4):493-509. doi: 10.1007/BF00927893.
In a study of the immune response of the rat to infection with the nematode Strongyloidis ratti, the antigens of the infective larval stage (L3) and of the parasitic, parthenogenetic female (Fp) were investigated. From both the larvae and the adult females, one metabolic (exoantigen) and two somatic antigens were extracted. Of the two somatic antigens, one was soluble and obtainable by physical means while the other was separated by chemical means from the tegument of the parasite. Humoral responses to the various antigens were evaluated by immunodiffusion and ELISA techniques, while the overall immune response was assayed by the worm burden in the immunized and subsequently infected rats. Agar-gel double diffusion yielded precipitin bands only with larval somatic antigens. ELISA proved positive at a titer of 20,000 with larval metabolic antigen and sera of rats immunized against either larval metabolic or somatic antigens. By 20 days post challenge infection, however, this titer diminished to 4000. In vivo studies of worm burden in rats immunized with the various antigens and then exposed to the live L3 of the nematode showed that there were significantly fewer adult worms in the rats immunized with larval somatic antigen and adult metabolic antigen than in those immunized with adult somatic antigen or larval metabolic antigen.
在一项关于大鼠对线虫类圆线虫感染的免疫反应研究中，对感染性幼虫阶段（L3）和寄生性孤雌生殖雌虫（Fp）的抗原进行了研究。从幼虫和成年雌虫中分别提取了一种代谢（外抗原）和两种体抗原。在这两种体抗原中，一种是可溶的，可通过物理方法获得，而另一种则通过化学方法从寄生虫的体表分离得到。通过免疫扩散和酶联免疫吸附测定（ELISA）技术评估了对各种抗原的体液反应，而通过免疫大鼠并随后感染大鼠后的虫负荷来测定总体免疫反应。琼脂凝胶双向扩散仅与幼虫体抗原产生沉淀带。ELISA检测显示，用幼虫代谢抗原以及针对幼虫代谢或体抗原免疫的大鼠血清，在滴度为20000时呈阳性。然而，在攻击感染后20天，该滴度降至4000。在用各种抗原免疫并随后接触线虫活L3的大鼠中进行的虫负荷体内研究表明，用幼虫体抗原和成年代谢抗原免疫的大鼠体内的成虫数量明显少于用成年体抗原或幼虫代谢抗原免疫的大鼠。
