Jager E, Rüden H, Zeschmar-Lahl B
Institut für Hygiene, Freien Universität Berlin.
Zentralbl Hyg Umweltmed. 1994 Dec;196(4):367-79.
At two composting facilities (plants D and E), contamination of the air with total bacteria and mould fungi, and in addition with gram-negative bacteria (only at plant D) was analyzed at different indoor sites and outdoor in the vicinity. Statistical validity of the determination of contents of microorganisms in air samples was guaranteed by the collection and analysis of 30 parallel samples. At plant D, total bacteria concentrations in outdoor air ranged from 106 to 15,618 CFU/m3 air (median: 495 CFU/m3 air), gram-negative bacteria concentrations ranged up to 71 CFU/m3 air (median: below the detection limit of 35 CFU/m3 air), and mould fungi reached 7,138 CFU/m3 air (median: 141 CFU/m3 air). Highest concentrations of total bacteria above the upper detection limit (> 84,806 CFU/m3 air, sample volume: 28.3 l) and of mould fungi (38,940 CFU/m3 air) occurred at the place where three months old compost was mixed, highest concentrations of gram-negative bacteria (14,134 CFU/m3 air) were measured during mixing of fresh compost (younger than 8 days). Maximum and median values of the examined microorganisms ranged so high that special protective means for personnel working directly beneath the mixing process seem to be necessary under hygienic aspects. Raw and clean air at the composting filter at plant D showed highly significant differences concerning air concentration of gram-negative bacteria and mould fungi, indicating a good separation efficiency for these types of microorganisms. Maximum and median values of gram-negative bacteria and mould fungi concentrations (all < 1,000 CFU/m3 air) measured in clean air behind the composting filter lie in the range of normal outdoor air. Merely total bacteria show statistical significant differences to outdoor air with median values of clean air of 1,979 CFU/m3 air (edge of filter) resp. 3,110 CFU/m3 air (upside the filter) and with maximum values of above 30,000 CFU/m3 air in each case. In the outdoor air at plant E, total bacteria concentrations ranged from 362 to 7,633 CFU/m3 air (median: 1,312 CFU/m3 air), mould fungi ranged from 115 to 4,072 CFU/m3 air (median: 345 CFU/m3 air). Highest concentrations of total bacteria and mould fungi (all analyses above the upper detection limit of 21,201 CFU/m3 air; sample volume: 113,2 l) occurred during shredding of a mixture of kitchen and green wastes and of shrubs. During experimental shredding of separately collected panty diapers air concentrations of total bacteria ranged from about 19,000 CFU/m3 air to > 21,201 CFU/m3 air.(ABSTRACT TRUNCATED AT 400 WORDS)
在两个堆肥设施(D厂和E厂),对不同室内场所及周边室外空气中的总细菌、霉菌以及革兰氏阴性菌(仅在D厂)污染情况进行了分析。通过采集和分析30个平行样本确保了空气样本中微生物含量测定的统计有效性。在D厂,室外空气中总细菌浓度范围为106至15,618 CFU/m³空气(中位数:495 CFU/m³空气),革兰氏阴性菌浓度最高达71 CFU/m³空气(中位数:低于35 CFU/m³空气的检测限),霉菌浓度达7,138 CFU/m³空气(中位数:141 CFU/m³空气)。总细菌浓度高于检测上限(> 84,806 CFU/m³空气,样本体积:28.3升)以及霉菌浓度(38,940 CFU/m³空气)的最高值出现在三个月龄堆肥混合处,革兰氏阴性菌浓度最高值(14,134 CFU/m³空气)出现在新鲜堆肥(小于8天)混合期间。所检测微生物的最大值和中位数范围如此之高,以至于从卫生角度来看,似乎有必要为在混合过程正下方工作的人员采取特殊防护措施。D厂堆肥过滤器处的原始空气和清洁空气在革兰氏阴性菌和霉菌的空气浓度方面存在高度显著差异,表明对这些类型的微生物具有良好的分离效率。在堆肥过滤器后的清洁空气中测得的革兰氏阴性菌和霉菌浓度的最大值和中位数(均< 1,000 CFU/m³空气)处于正常室外空气范围内。仅总细菌与室外空气存在统计学显著差异,清洁空气的中位数分别为1,979 CFU/m³空气(过滤器边缘)和3,110 CFU/m³空气(过滤器上方),且每种情况下最大值均高于30,000 CFU/m³空气。在E厂的室外空气中,总细菌浓度范围为362至7,633 CFU/m³空气(中位数:1,312 CFU/m³空气),霉菌浓度范围为115至4,072 CFU/m³空气(中位数:345 CFU/m³空气)。总细菌和霉菌浓度的最高值(所有分析均高于21,201 CFU/m³空气的检测上限;样本体积:113.2升)出现在厨房和绿色废物与灌木混合物的粉碎过程中。在对单独收集的纸尿片进行实验性粉碎时,空气中总细菌浓度范围约为19,000 CFU/m³空气至> 21,201 CFU/m³空气。(摘要截于400字)