Biomaterials Research Group, Department of Materials Engineering, Isfahan University of Technology, Isfahan 84156-83111, Iran.
Applied Physiology Research Center, Cardiovascular Research Institute, Department of Physiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan 81746-73461, Iran.
Int J Biol Macromol. 2021 Jun 1;180:311-323. doi: 10.1016/j.ijbiomac.2021.03.086. Epub 2021 Mar 15.
The study aims to develop a novel nanohybrid shear-thinning hydrogel with fast gelation, and variable mechanical and biological properties. This nanohybrid hydrogel was developed via self-assembly guest-host interaction between β-cyclodextrin modified alginate (host macromere, Alg-CD) and adamantine modified graphene oxide (guest macromere, Ad-GO) and subsequent ionic crosslinking process. We found that the rheological and mechanical properties of hydrogels were controlled via macromere concentration and the host: guest macromere ratio, due to the modulation of crosslinking density and network structure. Noticeably, 12%(1:2) dual-crosslinked hydrogel (2DC12) significantly improved the strength (1.3-folds) and toughness compared to 10%(1:4) dual-crosslinked hydrogel (4DC10). Furthermore, the hydrogel erosion and cytocompatibility relied on the designed parameters. Remarkably, 2DC12 showed less than 20% weight loss after 20 days of incubation in physiological solution and more than 200% cell survival after five days. In conclusion, the nanohybrid Alg-GO hydrogel could be used as an injectable hydrogel for soft tissue engineering applications.
本研究旨在开发一种具有快速凝胶化能力、可变机械性能和生物性能的新型纳米杂化剪切稀化水凝胶。该纳米杂化水凝胶是通过β-环糊精修饰的海藻酸钠（主链大分子，Alg-CD）与金刚烷修饰的氧化石墨烯（客链大分子，Ad-GO）之间的自组装主客体相互作用以及随后的离子交联过程开发的。我们发现，由于交联密度和网络结构的调节，水凝胶的流变和力学性能可以通过大分子浓度和主链大分子与客链大分子的比例来控制。值得注意的是，与 10%（1:4）双交联水凝胶（4DC10）相比，12%（1:2）双交联水凝胶（2DC12）显著提高了强度（提高了 1.3 倍）和韧性。此外，水凝胶的侵蚀和细胞相容性依赖于设计参数。值得注意的是，在生理溶液中孵育 20 天后，2DC12 的重量损失小于 20%，而在 5 天后细胞存活率超过 200%。总之，纳米杂化 Alg-GO 水凝胶可用作软组织工程应用的可注射水凝胶。
